Articles
COMMERCIAL-SCALE PHOTO-AUTOTROPHIC MICROPROPAGATION: APPLICATIONS IN IRISH AGRICULTURE, HORTICULTURE AND FORESTRY
Article number
440_90
Pages
515 – 520
Language
Abstract
Commercial-scale photoautotrophic micropropagation systems have been developed and applied in Ireland for the production of a wide range of ornamental plant species, disease-free potato foundation stock and the mass propagation of ‘elite’ clones of temperate broadleaf forestry species.
This paper describes the background to this work and the systems used.
An essential element of this work has been the optimisation of environmental regimes and controls with respect to temperature, humidity, light intensity and carbon dioxide enrichment.
Also of importance has been the development of modified nutrient delivery systems, growth substrates, and culture vessels to maximise production capacity and cost efficiency.
Simplification of tissue culture media and production techniques were also key factors in maintaining the commercial feasibility of this technology.
This paper describes the background to this work and the systems used.
An essential element of this work has been the optimisation of environmental regimes and controls with respect to temperature, humidity, light intensity and carbon dioxide enrichment.
Also of importance has been the development of modified nutrient delivery systems, growth substrates, and culture vessels to maximise production capacity and cost efficiency.
Simplification of tissue culture media and production techniques were also key factors in maintaining the commercial feasibility of this technology.
Authors
T.D. Roche, R.D. Long, A.J. Sayegh, M.J. Hennerty
Keywords
CO2-enrichment, contamination, environmental control, Solanum tuberosum, substrates
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