Articles
DISTRIBUTION OF FIVE PLUM POX POTYVIRUS STRAINS IN IN VITRO GF8-1 PLANTS AS A PREREQUISITE TO THE DEVELOPMENT OF PPV IN VITRO TESTS
Article number
550_11
Pages
97 – 102
Language
English
Abstract
With the intention of optimising the Plum pox potyvirus (PPV) in vitro inoculation test, we checked the distribution of 5 PPV strains –3 M type strains and 2 D type strains- in Marianna plum GF8-1 in vitro shoots.
Five cuttings of each in vitro GF8-1 main stem were checked for presence of the virus by immunoprinting.
Prints were scored 0 when no virus was detected on the print and up to 4 when the infection was generalised on the print area.
The results showed significantly different virus contamination incidents between the different levels of the in vitro plants, except for the 93.080 D+ type strain.
Virus distribution was different depending on strain.
Dideron (D) strain and 95.064 M type Turkish isolate were mainly concentrated in the lowest parts of the plant whereas Marcus (M) strain and 91.006 M type French isolate were mainly concentrated in the middle part of the plant.
A non-homogeneous strain-dependent distribution of PPV in in vitro GF8-1 plants was therefore observed, but the basal half of the vitro plant appeared to be more heavily infected.
This distribution will have to be taken into account when using infected GF8-1 shoot tip as an inoculum source in inoculation in vitro tests.
Five cuttings of each in vitro GF8-1 main stem were checked for presence of the virus by immunoprinting.
Prints were scored 0 when no virus was detected on the print and up to 4 when the infection was generalised on the print area.
The results showed significantly different virus contamination incidents between the different levels of the in vitro plants, except for the 93.080 D+ type strain.
Virus distribution was different depending on strain.
Dideron (D) strain and 95.064 M type Turkish isolate were mainly concentrated in the lowest parts of the plant whereas Marcus (M) strain and 91.006 M type French isolate were mainly concentrated in the middle part of the plant.
A non-homogeneous strain-dependent distribution of PPV in in vitro GF8-1 plants was therefore observed, but the basal half of the vitro plant appeared to be more heavily infected.
This distribution will have to be taken into account when using infected GF8-1 shoot tip as an inoculum source in inoculation in vitro tests.
Publication
Authors
M. Bodin Ferri, I. Constant, V. Schurdi-Levraud, F. Dosba
Keywords
Sharka, Shoot grafting, Inoculum, PPV strains, Micropropagation, In vitro culture
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