Articles
EUROPEAN SPINDLE TREE AND COMMON PRIVET A NEW NATURAL HOSTS OF PLUM POX VIRUS
Article number
550_17
Pages
125 – 128
Language
English
Abstract
One locality infected with PPV was found in the course of monitoring natural sources of Plum pox virus (PPV) in South Moravian region (Czech Republic). Wild growing plums and blackthorns were proved to be infected with PPV in this locality.
Moreover shrubs of Euonymus europea and Ligustrum vulgare revealed distinct diffuse spots and rings in leaves similar to those caused by PPV. Detection of PPV was carried out by ELISA and immunosorbent electron microscopy and confirmed infection with PPV not only in plums and blackthorns but also in leaves of both E. europea and L. vulgare. The virus was transmitted by mechanical inoculation from both investigated species to Chenopodium foetidum and Nicotiana benthamiana. Indirect ELISA using PPV-M monoclonal antibodies was negative and did not confirm presence of PPV-M strain in the locality.
Moreover shrubs of Euonymus europea and Ligustrum vulgare revealed distinct diffuse spots and rings in leaves similar to those caused by PPV. Detection of PPV was carried out by ELISA and immunosorbent electron microscopy and confirmed infection with PPV not only in plums and blackthorns but also in leaves of both E. europea and L. vulgare. The virus was transmitted by mechanical inoculation from both investigated species to Chenopodium foetidum and Nicotiana benthamiana. Indirect ELISA using PPV-M monoclonal antibodies was negative and did not confirm presence of PPV-M strain in the locality.
Publication
Authors
J. Polák
Keywords
Sharka, Natural infection, Ligustrum vulgare, Euonymus europea, Detection, ELISA, ISEM, Indicator plants
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