Articles
COMPARISON OF BIOASSAYS AND LABORATORY ASSAYS FOR APPLE STEM GROOVING VIRUS
Article number
550_40
Pages
281 – 284
Language
English
Abstract
The standard double-budding assay and the glasshouse test with the indicator Malus micromalus, were compared with ELISA and immunocapture PCR for the detection of Apple stem grooving virus (ASGV) in 102 apple trees and 3 oriental pear.
Twenty-two trees were positive for ASGV by both ELISA and IC-PCR but three of these trees were negative by Virginia Crab, three were negative by M. micromalus and one was negative by both these bioassays.
The infected trees were re-tested by IC-PCR and ELISA in a second year; the IC-PCR results were confirmed but two of the 22 infected trees were negative by ELISA. On this evidence, IC-PCR is a more reliable assay for ASGV than the slow and expensive bioassays.
Twenty-two trees were positive for ASGV by both ELISA and IC-PCR but three of these trees were negative by Virginia Crab, three were negative by M. micromalus and one was negative by both these bioassays.
The infected trees were re-tested by IC-PCR and ELISA in a second year; the IC-PCR results were confirmed but two of the 22 infected trees were negative by ELISA. On this evidence, IC-PCR is a more reliable assay for ASGV than the slow and expensive bioassays.
Publication
Authors
M.J. Kirby, C.M. Guise, A.N. Adams
Keywords
Field indexing, Glasshouse indexing, ELISA, Polymerase chain reaction, IC-PCR, Apple stem grooving virus, assays
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