Articles
VARIABILITY AND MOLECULAR TYPING OF THE WOODY-TREE INFECTING ILARVIRUSES (PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNE DWARF VIRUS)
Article number
550_33
Pages
237 – 244
Language
English
Abstract
Prunus necrotic ringspot virus (PNRSV) and Prune dwarf virus (PDV) (genus Ilarvirus, family Bromoviridae) are economically important worldwide distributed viruses of stone fruit trees.
In order to search for the bases of extensive variability of viroses caused by these pathogens, we analysed 16 RNA3 sequences of PNRSV and 11 of PDV isolates originating from central Europe.
These sequences were compared with previously published data (in case of PNRSV with 30 sequences). According to phylogenetic analyses, the PNRSV isolates split into three main groups which (to a certain extent) correlated with their geographic origin.
Modelled serological properties arranged all the new isolates to serotype CH9. For some of the isolates a classification to serotype CH30 was indicated.
An interesting phenomenon was observed in the distribution of the computed molecular masses of the putative translation products of the capsid protein gene.
The region situated downstream of the transcription start of RNA4 was the most variable part of RNA3. Comparison of all (13) sequenced isolates of PDV revealed high (88%) conservation of the capsid protein.
The highest degree of identity was observed in the C-terminal half.
No reasonable correlation between amino acid substitutions and host species and/or geographic origin of the isolates has been observed.
Alignment with capsid proteins of other ilarviruses revealed Apple mosaic virus, Elm mottle virus, Lilac ring mottle virus and Prunus necrotic ringspot virus as the most related viruses.
In order to search for the bases of extensive variability of viroses caused by these pathogens, we analysed 16 RNA3 sequences of PNRSV and 11 of PDV isolates originating from central Europe.
These sequences were compared with previously published data (in case of PNRSV with 30 sequences). According to phylogenetic analyses, the PNRSV isolates split into three main groups which (to a certain extent) correlated with their geographic origin.
Modelled serological properties arranged all the new isolates to serotype CH9. For some of the isolates a classification to serotype CH30 was indicated.
An interesting phenomenon was observed in the distribution of the computed molecular masses of the putative translation products of the capsid protein gene.
The region situated downstream of the transcription start of RNA4 was the most variable part of RNA3. Comparison of all (13) sequenced isolates of PDV revealed high (88%) conservation of the capsid protein.
The highest degree of identity was observed in the C-terminal half.
No reasonable correlation between amino acid substitutions and host species and/or geographic origin of the isolates has been observed.
Alignment with capsid proteins of other ilarviruses revealed Apple mosaic virus, Elm mottle virus, Lilac ring mottle virus and Prunus necrotic ringspot virus as the most related viruses.
Publication
Authors
D. Vasková, K. Petrzik, J. Spak, R. Karesová
Keywords
PNRSV, PDV, ApMV, Bromoviridae, molecular typing, RNA3
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