Articles
ISOLATION AND PARTIAL CHARACTERISATION OF A NEW VIRUS INFECTING PRUNUS ARMENIACA (APRICOT) AND BELONGING TO THE NEPOVIRUS GENUS
Article number
550_24
Pages
169 – 176
Language
English
Abstract
An extended survey of the sanitary status of Prunus orchards in the south-east of France lead us to collect several budwood samples from a commercial apricot orchard where trees displayed reduced vigour but had no obvious virus-like symptoms on leaves or fruits.
Indexing on GF305 peach seedlings in the greenhouse resulted in stunting and other symptoms reminiscent of a Nepovirus infection 1 month after inoculation.
The virus was successfully transmitted to herbaceous hosts and then purified using sucrose gradient centrifugation.
Under the electron microscope, purified viral particles displayed a typical Nepovirus morphology.
The particles contain two single stranded RNAs of 5.9 and 7.9 kb, and a single type of coat protein subunit of 53.7 kDa.
By serological analysis, no relationships were found with other Nepoviruses.
Partial sequence analysis related this new agent as a putative member of the Nepovirus group.
Taken together these results indicate clearly that the virus isolated is a seemingly undescribed Nepovirus. Given the lack of symptoms observed on the original host after back inoculation with purified viral particles, we provisionally named this virus Apricot latent ringspot virus (ALRSV).
Indexing on GF305 peach seedlings in the greenhouse resulted in stunting and other symptoms reminiscent of a Nepovirus infection 1 month after inoculation.
The virus was successfully transmitted to herbaceous hosts and then purified using sucrose gradient centrifugation.
Under the electron microscope, purified viral particles displayed a typical Nepovirus morphology.
The particles contain two single stranded RNAs of 5.9 and 7.9 kb, and a single type of coat protein subunit of 53.7 kDa.
By serological analysis, no relationships were found with other Nepoviruses.
Partial sequence analysis related this new agent as a putative member of the Nepovirus group.
Taken together these results indicate clearly that the virus isolated is a seemingly undescribed Nepovirus. Given the lack of symptoms observed on the original host after back inoculation with purified viral particles, we provisionally named this virus Apricot latent ringspot virus (ALRSV).
Publication
Authors
P. Gentit, R.P. Delbos, T. Candresse, J. Dunez
Keywords
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