Articles
BIOLOGICAL PROPERTIES AND PARTIAL MOLECULAR CHARACTERISATION OF TWO FOVEAVIRUSES ASSOCIATED WITH SIMILAR DISORDERS OF CHERRY TREES
Article number
550_23
Pages
161 – 168
Language
English
Abstract
A new cherry disorder discovered by Ctifl centre of Lanxade was indexed on different indicators in greenhouse and nursery.
According to the isolate, symptoms induced on GF305 peach seedlings were weak or severe.
GF305 infected by severe isolates showed large yellowish streaks or mottles inducing a deformation of foliage or sickle like arching.
This new disorder was transmitted to fruit and ornamental cherries and to other Prunus. Attempts to transmit this new pathogen to herbaceous hosts were unsuccessful, preventing us from purifying the viral agent.
However, dsRNAs could be purified from two severe isolates, named P1C124 and P2C124. Partials cDNAs were obtained from reverse transcription of P1C124 dsRNAs either by degenerated oligonucleotide-based PCR (DOP-PCR) or SMARTTM long-distance RT-PCR (LD-RT-PCR). All P1C124 cDNAs showed homology ranging from 73 to 90 % with the Sour cherry green ring mottle virus (SCGRMV) genome.
When total RNA extracts of GF305 seedlings graft-inoculated with SCGRMV, P1C124 and P2C124, were hybridised with a P1C124- specific RNA probe, only P1C124 and SCGRMV could be recognised at high hybridisation stringency.
Lower stringency were required to hybridise P2C124 and SCGRMV. Moreover, the sequence of a DOP-PCR cDNA obtained from P2C124 dsRNA, was only 66 % homologous to the SCGRMV corresponding sequence but shared 97 % homology with another cherry foveavirus recently characterised, the Cherry necrotic rusty mottle virus (CNRMV, M. Rott and W. Jelkmann, personal communication). The P2C124 3′ region encoding coat protein was cloned and confirmed that this viral isolate was more related to CNRMV than to SCGRMV. Greenhouse and nursery indexing on various Prunus hosts have been undertaken to compare the biological properties of these foveaviruses inducing disorders in cherry trees.
According to the isolate, symptoms induced on GF305 peach seedlings were weak or severe.
GF305 infected by severe isolates showed large yellowish streaks or mottles inducing a deformation of foliage or sickle like arching.
This new disorder was transmitted to fruit and ornamental cherries and to other Prunus. Attempts to transmit this new pathogen to herbaceous hosts were unsuccessful, preventing us from purifying the viral agent.
However, dsRNAs could be purified from two severe isolates, named P1C124 and P2C124. Partials cDNAs were obtained from reverse transcription of P1C124 dsRNAs either by degenerated oligonucleotide-based PCR (DOP-PCR) or SMARTTM long-distance RT-PCR (LD-RT-PCR). All P1C124 cDNAs showed homology ranging from 73 to 90 % with the Sour cherry green ring mottle virus (SCGRMV) genome.
When total RNA extracts of GF305 seedlings graft-inoculated with SCGRMV, P1C124 and P2C124, were hybridised with a P1C124- specific RNA probe, only P1C124 and SCGRMV could be recognised at high hybridisation stringency.
Lower stringency were required to hybridise P2C124 and SCGRMV. Moreover, the sequence of a DOP-PCR cDNA obtained from P2C124 dsRNA, was only 66 % homologous to the SCGRMV corresponding sequence but shared 97 % homology with another cherry foveavirus recently characterised, the Cherry necrotic rusty mottle virus (CNRMV, M. Rott and W. Jelkmann, personal communication). The P2C124 3′ region encoding coat protein was cloned and confirmed that this viral isolate was more related to CNRMV than to SCGRMV. Greenhouse and nursery indexing on various Prunus hosts have been undertaken to compare the biological properties of these foveaviruses inducing disorders in cherry trees.
Publication
Authors
P. Gentit, X. Foissac, L. Svanella-Dumas, M. Peypelut, G. Macquaire, T. Candresse
Keywords
Cherry, Foveavirus, Double-stranded RNAs, DOP-PCR
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