Articles
SIGNIFICANT RESISTANCE OF TRANSGENIC PLUMS AGAINST THE FOUR SEROTYPES OF PLUM POX POTYVIRUS
Article number
550_64
Pages
431 – 435
Language
English
Abstract
Transgenic plum C5 was reported as resistant to plum pox potyvirus (PPV) infection under confined greenhouse conditions.
To better characterize the resistance phenotype of this clone, challenging assays using the four major serotypes of PPV were conducted.
Observations through two dormancy cycles revealed that control plants (transgenic and non-transformed) were infected, however the transgenic plum C5 remained symptomless and DAS-ELISA and western-blotting negative.
A comparison of the nucleotide sequences of the capsid gene from these four PPV serotypes with that engineered into the plum genome led us to conclude that the highly conserved 2/3rd C-terminus sequence of CP cistron may be the basic motif involved in the homology-dependent gene resistance.
To better characterize the resistance phenotype of this clone, challenging assays using the four major serotypes of PPV were conducted.
Observations through two dormancy cycles revealed that control plants (transgenic and non-transformed) were infected, however the transgenic plum C5 remained symptomless and DAS-ELISA and western-blotting negative.
A comparison of the nucleotide sequences of the capsid gene from these four PPV serotypes with that engineered into the plum genome led us to conclude that the highly conserved 2/3rd C-terminus sequence of CP cistron may be the basic motif involved in the homology-dependent gene resistance.
Publication
Authors
M. Ravelonandro, P. Briard, R. Scorza
Keywords
Prunus domestica, transgenics, plum pox potyvirus, capsid gene
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