Home > XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops – Top Fruit Diseases > RESISTANCE TO PLUM POX POTYVIRUS IN A TRANSGENIC WOODY PERENNIAL FRUIT TREE, EUROPEAN PLUM (Prunus domestica L.) RESULTS FROM POST-TRANSCRIPTIONAL GENE SILENCING

Articles

RESISTANCE TO PLUM POX POTYVIRUS IN A TRANSGENIC WOODY PERENNIAL FRUIT TREE, EUROPEAN PLUM (Prunus domestica L.) RESULTS FROM POST-TRANSCRIPTIONAL GENE SILENCING

Article number

550_63

Pages

425 – 430

Language

English

Abstract

Transgenic plums containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV and displayed characteristics typical of post-transcriptional gene silencing (PTGS) as described in herbaceous species, including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, and a complex multicopy transgene insertion.

Publication

XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops – Top Fruit Diseases

Authors

R. Scorza, A.M. Callahan, L. Levy, V. Damsteegt, M. Ravelonandro

Keywords

RNA-mediated resistance, Transgene silencing, Post-transcriptional gene silencing

Full text

https://doi.org/10.17660/ActaHortic.2001.550.63

Online Articles (71)

2. ADVANCES IN THE METHODS OF PATHOGEN DETECTION-INTRODUCTORY REMARKS

T. Candresse

3. POLYVALENT DETECTION OF FRUIT TREE TRICHO, CAPILLO AND FOVEAVIRUSES BY NESTED RT-PCR USING DEGENERATED AND INOSINE CONTAINING PRIMERS (PDO RT-PCR)

X. Foissac | L. Svanella-Dumas | M.J. Dulucq | T. Candresse | P. Gentit

4. DEVELOPMENT OF ROUTINE RT-PCR ELOSA TESTS FOR FRUIT TREE CERTIFICATION

J. Kummert | M. Vendrame | P. Lepoivre | S. Steyer

5. DETECTION OF NEMATODE–TRANSMITTED NEPOVIRUSES BY THE NOVEL, ONE-TUBE AMPLIDET RNA ASSAY

M.M. Klerks | J.F.J.M. van den Heuvel | C.D. Schoen

6. SIMULTANEOUS DETECTION OF STONE OR POME FRUIT VIROIDS BY SINGLE TUBE-RT-PCR

F. Faggioli | E. Ragozzino | M. Barba

7. THE DIAGNOSIS OF PLUM POX VIRUS IN THE UK: FROM STRAIN DIFFERENTIATION TO ON-SITE DETECTION

R.A. Mumford | A.S. Perrin | C. Danks

8. CURRENT SITUATION OF PLUM POX DISEASE ON STONE FRUIT SPECIES IN MIDDLE AND EASTERN EUROPE

9. APHIDS CAN ACQUIRE PLUM POX VIRUS FROM INFECTED FRUITS

G. Labonne | J.B. Quiot

10. PLUM POX VIRUS DIVERSIFICATION OBSERVED IN A SMALL CLUSTER OF ORCHARDS

L. Quiot-Douine | M. Boeglin | S. Dallot | V. Marie-Jeanne | J.B. Quiot

11. MOLECULAR CHARACTERIZATION OF PLUM POX VIRUS ALMOND ISOLATE

D. Pribék | L. Palkovics | R. Gáborjányi

12. DISTRIBUTION OF FIVE PLUM POX POTYVIRUS STRAINS IN IN VITRO GF8-1 PLANTS AS A PREREQUISITE TO THE DEVELOPMENT OF PPV IN VITRO TESTS

M. Bodin Ferri | I. Constant | V. Schurdi-Levraud | F. Dosba

13. COMPARISON OF APRICOT GENOTYPES WITH DIFFERENT RESISTANCE LEVEL TO PLUM POX VIRUS (PPV)

E. Fuchs | M. Grüntzig | I. Ernst | H. Kegler

14. CHARACTERISATION OF APRICOT CULTIVARS INFECTED WITH PLUM POX VIRUS USING VIRUS CONCENTRATION IN LEAVES

I. Guillet-Bellanger | J.M. Audergon

15. INHERITANCE OF THE STARK EARLY ORANGE APRICOT CULTIVAR RESISTANCE TO PLUM POX VIRUS

I. Guillet-Bellanger | J.M. Audergon

16. STUDIES ON PLUM POX (SHARKA) RESISTANCE IN APRICOT

T.A. Moustafa | M.L. Badenes | J. Martínez-Calvo | G. Liacer

17. LONG TERM EVALUATION OF PLUM CULTIVARS ARTIFICIALLY INFECTED BY PPV

F. Paprstein | R. Karesová

18. EUROPEAN SPINDLE TREE AND COMMON PRIVET A NEW NATURAL HOSTS OF PLUM POX VIRUS

J. Polák

19. MONITORING AND SPATIOTEMPORAL ANALYSIS OF PPV-M SPREAD IN TWO APRICOT ORCHARDS IN SOUTHERN GREECE

C. Varveri | E. Zintzaras | D. Dimou | B. Di Terlizzi

20. NEW INSIGHTS ON PLUM POX VIRUS PRESENT IN CHILE

21. FIRST RECORD OF PLUM POX VIRUS IN JORDAN

M. Al Rwahnih | D. Boscia | A. Myrta | B. Di Terlizzi

22. PRELIMINARY CHARACTERIZATION OF A NORTH AMERICAN ISOLATE OF PLUM POX VIRUS FROM NATURALLY INFECTED PEACH AND PLUM ORCHARDS IN PENNSYLVANIA, USA

23. WORKSHOP ON PLUM POX

M. Kölber

24. BIOLOGICAL PROPERTIES AND PARTIAL MOLECULAR CHARACTERISATION OF TWO FOVEAVIRUSES ASSOCIATED WITH SIMILAR DISORDERS OF CHERRY TREES

25. ISOLATION AND PARTIAL CHARACTERISATION OF A NEW VIRUS INFECTING PRUNUS ARMENIACA (APRICOT) AND BELONGING TO THE NEPOVIRUS GENUS

P. Gentit | R.P. Delbos | T. Candresse | J. Dunez

26. DIFFERENTIATION AMONG ISOLATES OF PRUNUS NECROTIC RINGSPOT VIRUS BY SINGLE- AND DOUBLE-STRANDED TRANSCRIPT CONFORMATION POLYMORPHISM

A. Rosner | L. Maslenin | S. Spiegel

27. DETECTION AND DIFFERENTIATION OF CHERRY MOTTLE LEAF VIRUS AND PEACH MOSAIC VIRUS

D. James | C. Upton

28. IDENTIFICATION OF A SECOND CLOSTEROVIRUS ASSOCIATED WITH LITTLE CHERRY DISEASE, LITTLE CHERRY VIRUS –2

M.E. Rott | W. Jelkmann

29. DEVELOPMENT OF PCR PRIMER PAIRS FOR THE CHARACTERIZATION AND DETECTION OF SEVERAL RELATED FILAMENTOUS VIRUSES OF CHERRY

M.E. Rott | W. Jelkmann

30. COMPLETE NUCLEOTIDE SEQUENCE OF CHERRY NECROTIC RUSTY MOTTLE VIRUS

M.E. Rott | W. Jelkmann

31. VARIANTS OF APRICOT LATENT FOVEAVIRUS (ALV) ISOLATED FROM SOUTH EUROPEAN ORCHARDS ASSOCIATED WITH PEACH ASTEROID SPOT AND PEACH SOOTY RINGSPOT DISEASES

P. Gentit | X. Foissac | L. Svanella-Dumas | T. Candresse

32. AMERICAN PLUM LINE PATTERN VIRUS IS A DISTINCT ILARVIRUS

S.W. Scott | M.T. Zimmerman

33. THE INTERACTION BETWEEN PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNE DWARF VIRUS IN PEACH STUNT DISEASE

S.W. Scott | M.T. Zimmerman | S. Yilmaz | E.I. Zehr | E. Bachman

34. VARIABILITY AND MOLECULAR TYPING OF THE WOODY-TREE INFECTING ILARVIRUSES (PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNE DWARF VIRUS)

D. Vasková | K. Petrzik | J. Spak | R. Karesová

35. VIRUSES OF STONE FRUITS IN PALESTINE

S. Jarrar | A. Myrta | B. Di Terlizzi | V. Savino

36. STUDIES ON THE AETIOLOGY OF APRICOT ‘VIRUELA’ DISEASE

M.C. Cañizares | F. Aparicio | K. Amari | V. Pallás

37. APPLE CHLOROTIC LEAF SPOT VIRUS IN GERMPLASM COLLECTION OF FRUIT SPECIES

R. Karesová | F. Paprstein

38. HISTOLOGICAL MANIFESTATION OF RUBBERY WOOD SYMPTOM IN APPLE TREES

R. Fialová | M. Navrátil | R. Podsedníková

39. THE USE OF A SIMPLIFIED DAS-ELISA PROCEDURE FOR LARGE-SCALE DETECTION OF APPLE CHLOROTIC LEAF SPOT AND APPLE STEM GROOVING VIRUSES IN APPLE TREES OVER EXTENDED SAMPLING PERIODS

L.M. Corvo | M.T.F. Barros

40. TO THE DIAGNOSIS AND DISTRIBUTION OF THE APPLE STEM PITTING VIRUS IN THE CZECH REPUBLIC

J.K. Kundu

41. COMPARISON OF BIOASSAYS AND LABORATORY ASSAYS FOR APPLE STEM GROOVING VIRUS

M.J. Kirby | C.M. Guise | A.N. Adams

42. GENOME HETEROGENEITY OF APPLE STEM PITTING VIRUS IN APPLE TREES

43. CHARACTERIZATION OF HUNGARIAN ISOLATES OF APPLE CHLOROTIC LEAF SPOT VIRUS

L. Krizbai | I. Ember | M. Németh | M. Kölber

44. INTRODUCTORY REMARKS TO THE SESSION “ETIOLOGY OF VIRUS AND VIROID DISEASES OF FRUIT TREES”

R. Flores

45. IDENTIFICATION OF GRAFT-TRANSMISSIBLE AGENTS FROM ‘TA TAO 5’ PEACH AND THEIR EFFECTS ON ‘CORONET’ PEACH

P.G. Gibson | G.L. Reighard | S.W. Scott | M.T. Zimmerman

46. TRACKING HOP STUNT VIROID INFECTION IN APRICOT TREES DURING A WHOLE YEAR BY NON-ISOTOPIC TISSUE PRINTING HYBRIDISATION

47. DETECTION OF VIROID-LIKE RNAS IN POMEGRANATE (PUNICA GRANATUM L.)

G. Gómez | V. Pallás

48. ATTEMPTS TO OBTAIN VIROID-FREE HOP PLANTS IN POLAND

E. Solarska | M. Grudzinska

49. THE FIELD DISTRIBUTION OF HOP LATENT VIROID WITHIN POLISH HOP CULTIVARS

E. Solarska | M. Grudzi&nacute;ska

50. TRANSMISSION BY PATCH GRAFTING OF ESFY PHYTOPLASMA TO APRICOT (PRUNUS ARMENIACA L) AND JAPANESE PLUM (PRUNUS SALICINA LINDL)

51. FRUIT TREE PHYTOPLASMA DISEASES DIFFUSED IN NATURE BY PSYLLIDS

L. Carraro | R. Osler | N. Loi | P. Ermacora | E. Refatti

52. PEACH ROSETTE, LITTLE PEACH, AND RED SUTURE ARE DISEASES INDUCED BY A PHYTOPLASMA CLOSELY RELATED TO WESTERN X-DISEASE

S.W. Scott | M.T. Zimmerman

53. ISOLATION AND EXPRESSION OF AN IMMUNODOMINANT MEMBRANE PROTEIN GENE OF THE ESFY PHYTOPLASMA FOR ANTISERUM PRODUCTION

E. Mergenthaler | O. Viczián | M. Fodor | S. Süle

54. EPIDEMIOLOGICAL STUDIES ON PEAR DECLINE (PD) IN ITALY

C. Poggi Pollini | R. Bassini | S. Guerrini | L. Giunchedi | G. Firrao

55. PHYTOPLASMA INFECTION IN PEACH AND CHERRY IN ITALY

56. A MOLECULAR SURVEY TO IDENTIFY PHYTOPLASMAS ASSOCIATED WITH APPLE TREES SHOWING DIFFERENT DISEASES SYMPTOMS

57. SURVEY FOR STONE FRUIT PHYTOPLASMAS IN THE CZECH REPUBLIC

58. IDENTIFICATION OF PHYTOPLASMAS INFECTING SOUR CHERRY IN HUNGARY

59. EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMAS IN SOUTHERN ENGLAND

D.L. Davies | A.N. Adams

60. ELABORATING CELL-SPECIFIC RESISTANCE TO PLUM POX POTYVIRUS

V. Decroocq | I. Carrère | I. Heinemann | M. Lansac | M. Ravelonandro

61. MOLECULAR VARIATION IN IMMUNODOMINANT MEMBRANE PROTEINS FROM PHYTOPLASMAS

D.J. Barbara | A. Morton | M.F. Clark | D.L. Davies

62. EXPRESSION OF THE IMMUNODOMINANT MEMBRANE PROTEIN OF CHLORANTIE-ASTER YELLOWS PHYTOPLASMA IN NICOTIANA BENTHAMIANA FROM A POTATO VIRUS X-BASED VECTOR

63. BACTERIAL SEQUENCES INTERFERING IN DETECTION OF PHYTOPLASMA BY PCR USING PRIMERS DERIVED FROM THE RIBOSOMAL RNA OPERON

L.J. Skrzeczkowski | W.E. Howell | KC. Eastwell | T.D. Cavileer

64. RESISTANCE TO PLUM POX POTYVIRUS IN A TRANSGENIC WOODY PERENNIAL FRUIT TREE, EUROPEAN PLUM (Prunus domestica L.) RESULTS FROM POST-TRANSCRIPTIONAL GENE SILENCING

R. Scorza | A.M. Callahan | L. Levy | V. Damsteegt | M. Ravelonandro

65. SIGNIFICANT RESISTANCE OF TRANSGENIC PLUMS AGAINST THE FOUR SEROTYPES OF PLUM POX POTYVIRUS

M. Ravelonandro | P. Briard | R. Scorza

66. INTRODUCTORY REMARKS TO THE SESSION ‘QUARANTINE THERAPY AND VIRUS-TESTED MATERIAL’

D. James

67. ADVANCES IN QUARANTINE TESTING OF TEMPERATE FRUIT TREE GERMPLASM AT USDA

G.R. Kinard | H.E. Waterworth | R.G. Mock

68. RECOVERY OF VIRUS-FREE ALMOND PLANTS BY IMPROVED IN VITRO SHOOT-TIP GRAFTING

A. Rizqi | M. Zemzami | S. Spiegel

69. HEAT TREATMENT, CHEMO-THERAPY AND HYDROPONIC CULTURE FOR OBTAINING VIRUS-FREE TREES OF SWEET CHERRY

W.E. Howell | K.C. Eastwell | T.S.C. Li

70. LONG TERM ASSESSMENT OF THE EFFECTS OF IN VITRO CHEMOTHERAPY AS A TOOL FOR APPLE STEM GROOVING VIRUS ELIMINATION

D. James

71. IMPROVED DETECTION OF VIRUSES AND PHYTOPLASMAS IN FRUIT TREE TISSUE CULTURES