Articles
POLYMORPHISM OF RIBES GENOTYPES USED IN POLISH BREEDING PROGRAM
Article number
585_17
Pages
125 – 132
Language
English
Abstract
Forty five Ribes genotypes of black currant, red currant and gooseberry, used frequently in the breeding program at the Research Institute of Pomology and Floriculture, were analysed by RAPD-PCR with sixty arbitrary sequenced, decamer primers (OPA, OPB and OPC groups; Operon Technologies). Among DNA fragments amplified in PCR, twenty eight (black currant), twenty three (red currant) and fourteen (gooseberry) were admitted as informative, polymorphic products, which allowed the discrimination of all analysed genotypes.
Use of primers OPA-02, -10, OPB-08, OPB-11, OPC-02 (black currant), OPA-02, -10, OPC-02, -06 (red currant) and OPC-02, -07, -08, -11 (gooseberry) allows us to obtain DNA patterns, typical for each of analysed genotype (“fingerprints”). Based on computer analysis of collected data, the dendrograms representing the relationship of Ribes genotypes used for crossing were created.
Use of primers OPA-02, -10, OPB-08, OPB-11, OPC-02 (black currant), OPA-02, -10, OPC-02, -06 (red currant) and OPC-02, -07, -08, -11 (gooseberry) allows us to obtain DNA patterns, typical for each of analysed genotype (“fingerprints”). Based on computer analysis of collected data, the dendrograms representing the relationship of Ribes genotypes used for crossing were created.
Publication
Authors
A. Kuras, M. Korbin, S. Pluta
Keywords
Ribes, RAPD-PCR, identification
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