Articles
PRELIMINARY STUDY ON ITS SEQUENCING AND CHARACTERIZATION OF ERIOBOTRYA
Article number
887_12
Pages
85 – 88
Language
English
Abstract
The nrDNA ITS gene fragments from ten Eriobotrya accessions (species, varieties, and types) were amplified with the primers ITSF 5-AAAAG TCGTAACAAGGTTTCC-3 and ITSR 5-GCTTAAATTCAGCGGGTAA-3. Results showed that the length of ITS of different Eriobotya plants ranged from 581 bp in E. malipoensis Kuan to 598 bp in E. bengalensis Hook.f. forma angustifolia Vidal, which means that ITS sequences were very conserved in length because of its low evolution rate.
The length of ITS1, 5.8s and ITS2 ranged between 212~223 bp, 166 bp and 203~209 bp respectively, among them 5.8s sequence was conservative.
The content of G+C of ITS sequence ranged from 64.02 to 65.71% and no significant differences were observed.
A total of 130 sites were found to be variable sites, in which 45 were located in ITS1, 76 at ITS2, and 5.8s have 9. The base mutation including G-A, C-T substitution, C-G, T-A transvertion, insertion or deletion showed by the ITS sequence could provide rich genetic information for phylogenetic and evolutionary research.
The length of ITS1, 5.8s and ITS2 ranged between 212~223 bp, 166 bp and 203~209 bp respectively, among them 5.8s sequence was conservative.
The content of G+C of ITS sequence ranged from 64.02 to 65.71% and no significant differences were observed.
A total of 130 sites were found to be variable sites, in which 45 were located in ITS1, 76 at ITS2, and 5.8s have 9. The base mutation including G-A, C-T substitution, C-G, T-A transvertion, insertion or deletion showed by the ITS sequence could provide rich genetic information for phylogenetic and evolutionary research.
Publication
Authors
X.H. Yang, S.Q. Lin, G.B. Hu, P. Li, S.J. Xu
Keywords
Eriobotrya, internal transcribed spacers, characterization
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