Articles
BIOCHEMICAL CHANGES DURING DEVELOPMENT PROCESS OF ANTHER-DERIVED EMBRYOS IN LOQUAT
Article number
887_35
Pages
215 – 219
Language
English
Abstract
The main biochemical changes during the development process of anther-derived embryos in loquat (Eriobotrya japonica Lindl. Dawuxing) were studied, including soluble proteins, nucleic acids, and carbohydrates.
The objectives were to explore the influential factors and molecular mechanism during the development process of anther-derived embryos and ultimately improve the plant regeneration system from anther culture in loquat.
At the important development turning points, soluble protein, soluble sugars, starch and nucleic acid changed significantly in metabolism.
During the development of loquat anther embryos, the dynamics of protein synthesis were roughly in S shape.
The two accumulation peaks appeared at globular stage and cotyledon stage respectively.
Alkaline protein contents were higher than acidic protein contents.
And alkaline protein contents and total soluble protein contents had the same change trend during the development process.
DNA contents and total nucleic acid contents had the same change trend during the development of anther-derived embryos.
The DNA synthesis peaks appeared at the embryogenic callus stage and differentiation stage.
RNA contents were very low at embryogenic callus stage and cotyledon stage, while DNA was actively synthesized at the two stages.
Soluble sugar, fructose and starch contents had the same change trend.
The two accumulation peaks appeared at globular stage and cotyledon stage, in accordance with the change of soluble protein.
The objectives were to explore the influential factors and molecular mechanism during the development process of anther-derived embryos and ultimately improve the plant regeneration system from anther culture in loquat.
At the important development turning points, soluble protein, soluble sugars, starch and nucleic acid changed significantly in metabolism.
During the development of loquat anther embryos, the dynamics of protein synthesis were roughly in S shape.
The two accumulation peaks appeared at globular stage and cotyledon stage respectively.
Alkaline protein contents were higher than acidic protein contents.
And alkaline protein contents and total soluble protein contents had the same change trend during the development process.
DNA contents and total nucleic acid contents had the same change trend during the development of anther-derived embryos.
The DNA synthesis peaks appeared at the embryogenic callus stage and differentiation stage.
RNA contents were very low at embryogenic callus stage and cotyledon stage, while DNA was actively synthesized at the two stages.
Soluble sugar, fructose and starch contents had the same change trend.
The two accumulation peaks appeared at globular stage and cotyledon stage, in accordance with the change of soluble protein.
Publication
Authors
R.Z. Gu, Y.Q. Wang, H.M. Qin, J. Yan
Keywords
anther culture, Eriobotrya japonica, haploidy
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