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Articles

FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN LOQUAT

Article number
887_49
Pages
285 – 290
Language
English
Abstract
The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars.
Protocols were developed for isolation and culture of loquat microspores.
Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures.
After 4 weeks of culture, the responding microspores produced yellowish/white callus.
The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.

Publication
III International Symposium on Loquat
Authors
D. Padoan, B. Chiancone, M.A. Germanà, P.S.S.V. Khan, I. Barany, M.C. Risueño, P.S. Testillano
Keywords
Eriobotrya japonica, cell structure, haploid, pollen embryogenesis
Full text
https://doi.org/10.17660/ActaHortic.2011.887.49
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