Articles
A RAPID AND EFFICIENT AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR LILIUM
Article number
900_19
Pages
161 – 167
Language
English
Abstract
A protocol has been developed for the clonal propagation and Agrobacterium-mediated transformation of the Lilium oriental hybrid Star Gazer. Transient GUS expression in basal plate disc-derived tissues and regenerated shoots was achieved following transformation using A. tumefaciens strain AGL1 containing pBI121, the latter carrying the β-glucuronidase (gus) and neomycin phosphotransferase II (nptII) genes.
A mean transformation efficiency of 20% was achieved in three independent transformation experiments.
Stable transgene integration and expression were confirmed by PCR and RT-PCR analyses.
The efficiency, reproducibility and rapidity of this protocol make it suitable for commercial application and for future research on this important ornamental plant.
A mean transformation efficiency of 20% was achieved in three independent transformation experiments.
Stable transgene integration and expression were confirmed by PCR and RT-PCR analyses.
The efficiency, reproducibility and rapidity of this protocol make it suitable for commercial application and for future research on this important ornamental plant.
Publication
Authors
F.F. Núñez de Cáceres, M.R. Davey, Z.A. Wilson
Keywords
Agrobacterium tumefaciens, β-glucuronidase (GUS), neomycin phosphotransferase II (nptII), Lilium ‘Star Gazer’
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