Articles
RESPONSE TO IN VITRO PROPAGATION OF DIFFERENT ASIATIC HYBRID LILIES
Article number
900_49
Pages
393 – 399
Language
English
Abstract
In vitro bulb scales of seven Asiatic hybrid lily (Lilium × elegans Thunb.) lines (12, 20, 599, 409, 00144, 00150 and 00151), differing for scale size (large and small), were cultured on MS medium supplemented with 8.8 µM BA and 1.1 µM NAA to induce adventitious shoots formation.
After 6 weeks, the frequency of shoot formation was very high in almost all genotypes (>94%), new shoots were obtained from the basal part of bulb scales and became shoot clusters.
These shoot clusters were cut into sections of about 4-7 mm in size at random and then cultured on the same medium for 6 weeks.
During the first and second subculture period, bulblets formed on large explants were greater and formed more shoots than small ones.
In the third subculture different combinations of BA (2.2-8.8-17.6 µM) and NAA (1.1-2.2 µM) were used to examine the effect on shoot cluster regeneration.
The media supplemented with 2.2 or 8.8 μM BA and 2.2 μM NAA were the most effective to promote shoot proliferation, shoot length and root growth, moreover the medium supplemented with 8.8 μM BA and 2.2 μM NAA was most effective to increase the biomass.
In all subcultures, the hybrids 00150, 00144 and 20 showed the highest values of shoot number, fresh weight, dry weight, shoot length and growth index.
Going from first to third subculture, shoot cluster cycle method improved adventitious shoot formation and increased the biomass.
All plantlets were successfully acclimated under greenhouse conditions.
After 6 weeks, the frequency of shoot formation was very high in almost all genotypes (>94%), new shoots were obtained from the basal part of bulb scales and became shoot clusters.
These shoot clusters were cut into sections of about 4-7 mm in size at random and then cultured on the same medium for 6 weeks.
During the first and second subculture period, bulblets formed on large explants were greater and formed more shoots than small ones.
In the third subculture different combinations of BA (2.2-8.8-17.6 µM) and NAA (1.1-2.2 µM) were used to examine the effect on shoot cluster regeneration.
The media supplemented with 2.2 or 8.8 μM BA and 2.2 μM NAA were the most effective to promote shoot proliferation, shoot length and root growth, moreover the medium supplemented with 8.8 μM BA and 2.2 μM NAA was most effective to increase the biomass.
In all subcultures, the hybrids 00150, 00144 and 20 showed the highest values of shoot number, fresh weight, dry weight, shoot length and growth index.
Going from first to third subculture, shoot cluster cycle method improved adventitious shoot formation and increased the biomass.
All plantlets were successfully acclimated under greenhouse conditions.
Publication
Authors
C.M. Cardona Suárez, G. Colla, M. Cardarelli, B. Nesi
Keywords
bulblet regeneration, shoot formation, shoot clusters, explant size
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