TOWARDS IN VITRO SELECTION FOR SALINITY TOLERANCE IN APRICOT CULTIVAR ‘CANINO’

In vitro mutation method was used to obtain salt tolerant apricot lines.
Small propagules of ‘Canino’ apricot were irradiated with the gamma ray at dose 0, 10, 25, 35, 50, 75 and 100 Gy.
After 30 days post treatment, post-irradiation recovery was assessed as the number of proliferated shoots per explants, fresh weight of cultures, and shoot length.
A sudden and sharp decrease in the survival percentage was noticed at75 Gy and complete lethality at 100 Gy.
A marked decline in number of regenerated shoots per explants and fresh weight of produced cultures was associated with lower irradiation doses.
Doses in the range of 10-75 Gy, which preserved survival was suitable for the induction of mutations in ‘Canino’ apricot cultivar.
Irradiated shoots were exposed to 25, 50, 75, 100, 125 mM NaCl added to the multiplication medium.
The number of vigorously growing shoots formed
30 days upon salinity treatments was determined.
Salt tolerant shoots were selected for cultivation and further analysis.
The utility of gamma ray induced mutagenesis for selecting salt tolerant apricot cultivars is discussed.

VII International Symposium on In Vitro Culture and Horticultural Breeding

A.S. El-sabagh, M.N. Barakat, E.A.-E. Genaidy

propagation, gamma ray mutation, salt tolerance IBA, BAP, M₃, MS₃ medium

https://doi.org/10.17660/ActaHortic.2012.961.16