Home > VII International Symposium on In Vitro Culture and Horticultural Breeding > IN VITRO CALLOGENESIS AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF GLOBE ARTICHOKE

Articles

IN VITRO CALLOGENESIS AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF GLOBE ARTICHOKE

Article number

961_34

Pages

267 – 271

Language

English

Abstract

Micropropagation techniques have been widely applied in globe artichoke
(C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far.
We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type Romanesco; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter.
After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.

Publication

VII International Symposium on In Vitro Culture and Horticultural Breeding

Authors

B. Menin, A. Moglia, C. Comino, S. Lanteri, T.W.J.M. Van Herpen, J. Beekwilder

Keywords

globe artichoke, callus culture, genetic transformation, Agrobacterium tumefaciens

Full text

https://doi.org/10.17660/ActaHortic.2012.961.34

Online Articles (77)

1. IN VITRO BREEDING FACILITATES CONVENTIONAL BREEDING FOR SCION AND ROOTSTOCK IMPROVEMENT IN CITRUS

J.W. Grosser | M. Calović | F.G. Gmitter Jr.

2. IN VITRO STORAGE AND CRYOPRESERVATION AS SUBSTANTIAL COMPLEMENTS IN CONCERTED ACTIONS TO BETTER MAINTAIN AND USE CROP GERMPLASM

E.R.J. Keller | B. Panis | F. Engelmann

3. DEVELOPMENT AND EVALUATION OF A NON-GMO BREEDING TECHNIQUE EXEMPLIFIED BY KALANCHOË

H. Lütken | E.B. Jensen | S.V. Wallström | R. Müller | B. Christensen

4. PRELIMINARY RESULTS ON THE USE OF MELIA AZEDARACH FRUIT EXTRACTS AS ANTIMICROBIAL AGENTS IN THE MICROPROPAGATION OF ZANTEDESCHIA

G. Marino | F. Gaggìa | B. Ruffoni

5. EXTENSIVE CITRUS TRIPLOID BREEDING PROGRAM BY 2X×4X SEXUAL HYBRIDIZATIONS

6. EVALUATION OF THE AFLP AND MSAP METHODS AS TOOLS FOR STUDYING OF DNA CHANGES IN GRAPEVINE PLANTS LONG PERIOD AFTER THEIR IN VITRO THERMOTHERAPY

M. Baranek | E. Ondrušíková

7. CONTAMINATION DURING MICROPROPAGATION: ANALYSIS OF THE BACTERIAL LINES AND TREATMENT WITH SAGE EXTRACT

8. IN VITRO CONSERVATION OF ORNAMENTAL PLANTS BY SLOW GROWTH STORAGE

C. Benelli | E.A. Ozudogru | M. Lambardi | G. Dradi

9. OPTIMISATION OF SOMATIC HYBRIDISATION IN CICHORIUM SPECIES

D. Deryckere | T. Eeckhaut | J. Van Huylenbroeck | E. Van Bockstaele

10. ORNAMENTAL PLANT TISSUE CULTURE INDUSTRY IN FLANDERS (BELGIUM)

E. Dhooghe | J. Van Huylenbroeck | D. Geelen

11. A PRELIMINARY INVESTIGATION INTO HOW PLANT GROWTH PROMOTING RHIZOBACTERIA CAN INFLUENCE THE FREQUENCY OF GENE TRANSFER IN AGROBACTERIUM– AND TRANSBACTER^TM-MEDIATED TRANSFORMATION SYSTEMS

B. Doyle Prestwich | O. O'Herlihy

12. EXPRESSION OF REGENERATION COMPETENCE FOR ADVENTITIOUS BUDDING, ROOTING AND SOMATIC EMBRYOGENESIS COMPARING TEMPERATE FRUIT TREE SPECIES

PH. Druart | K. Ben Mahmoud | S. Richet | V. Kondakhova

13. PROTOCOL DEVELOPMENT FOR IN VITRO ASSESSMENT OF CADMIUM TOLERANCE IN BLACK ALDER AND BASKET WILLOW AT THE CALLUS AND WHOLE PLANT LEVELS

M. Bajji | PH. Druart

14. IN VITRO PICLORAM-INDUCED SOMATIC EMBRYOGENESIS FROM LEAFLET OF PRUNUS INCISE AND EXPRESSION PROFILE OF CDC2 GENE

15. CHRYSANTHEMUM INDICUM PROTOPLAST CALLUS INDUCTION AND CULTURE

T. Eeckhaut | J. Van Huylenbroeck

16. TOWARDS IN VITRO SELECTION FOR SALINITY TOLERANCE IN APRICOT CULTIVAR ‘CANINO’

A.S. El-sabagh | M.N. Barakat | E.A.-E. Genaidy

17. DETERMINATION OF THE OPTIMUM MICROSPORE DEVELOPMENT STAGE AND OPTIMUM CULTURE MEDIUM IN ASPARAGUS (ASPARAGUS OFFICINALIS VAR. ALTILIS L.) FOR ANTHER CULTURE

N. Ercan | F. Ayar Şensoy

18. PLANT BIODIVERSITY AT HIGH ALTITUDE: IN VITRO PRESERVATION

P. Fasciani | G. Pirone | L. Pace

19. AN IMPROVED MICRO PROPAGATION SYSTEM FOR SUCCESSFUL TRANSFORMATION OF CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS)

F.G. Al-Swedi | S. Lane | M.P. Fuller

20. RETROTRANSPOSITION EVENTS IN BARLEY CALLUS CULTURES

A. Temel | N. Gozukirmizi

21. TOWARDS PHENOTYPING HEAT TOLERANCE IN GARDEN ROSES

O.F.C. Greyvenstein | T.W. Starman | H.B. Pemberton | G. Niu | D.H. Byrne

22. EFFECT OF GA₃ AND PACLOBUTRAZOL ON ADVENTITIOUS SHOOT REGENERATION OF TWO PELARGONIUM SP.

23. PRODUCTION OF TRANSGENIC APPLE (MALUS DOMESTICA BORKH.) FOR IMPROVEMENT OF FUNGAL RESISTANCE

N.M. Ali Bacha | A.A. Kader | H.-J. Jacobsen | F. Hassan

24. USE OF AFLP-MARKERS FOR ESTIMATION OF THE INBREEDING LEVEL IN HELLEBORUS ORIENTALIS

R.J. Heinrich | F. Klein | A. Hohe

25. CLONAL FIDELITY OF CHRYSANTHEMUM CULTIVARS AFTER LONG TERM MICROPROPAGATION BY STEM SEGMENT CULTURE

26. GENETIC STABILITY IN MICRO-CLONES OF ‘WOOD-APPLE’ DERIVED FROM DIFFERENT PATHWAYS OF MICROPROPAGATION AS REVEALED BY RAPD AND ISSR MARKERS

P. Joshi | S.D. Purohit

27. EFFECT OF GENOTYPE ON ORGANOGENESIS IN SIX GRAPE ROOTSTOCKS

B. Křižan | E. Ondrušiková | J. Moudrá | M. Pidra

28. EMBRYOGENIC CALLUS FORMATION FROM THE PETIOLES OF SPATHIPHYLLUM WALLISII

P.S. Lakshmanan | T. Eeckhaut | J. Van Huylenbroeck | E. Van Bockstaele

29. POLYPLOIDY BREEDING IN VITRO: EXPERIENCES WITH ORNAMENTALS

30. MODIFICATIONS IN LEAF ANATOMY OF BANANA PLANTS CULTIVAR ‘MAÇÃ’ SUBJECTED TO DIFFERENT SILICON SOURCES IN VITRO

31. MICROPROPAGATION AND IN VITRO GRAFTING TECHNIQUES TO ASSIST THE SELECTION OF A PISTACHIO ROOTSTOCK FROM A POPULATION OF TEREBINTH (PISTACIA TEREBINTHUS L.) COLLECTED IN THE SE OF SPAIN

32. EMBRYO RESCUE TO OVERCOME BARRIERS IN THE INTERSPECIFIC CROSS OF HELLEBORUS × HYBRIDUS AND H. NIGER

J. Meiners | T. Winkelmann

33. GLOBE ARTICHOKE CALLUS AS AN ALTERNATIVE SYSTEM FOR THE PRODUCTION OF DICAFFEOYLQUINIC ACIDS

A. Moglia | B. Menin | C. Comino | S. Lanteri | J. Beekwilder

34. IN VITRO CALLOGENESIS AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF GLOBE ARTICHOKE

35. CALLUS INDUCTION OF STEMONA CURTISII HOOK. F.

N. Montri | S. Chaisriha | C. Suwanphakdee

36. GROWING SPATHIPHYLLUM IN VITRO: EVALUATION OF SOME COMBINATIONS OF CARBOHYDRATE SOURCES AND MINERALS IN THE MEDIA REGARDING PEROXIDASE ENZYME ACTIVITY AND CHLOROPHYLL CONTENT

I.D. Mosonyi | A. Tilly-Mándy | E. Stefanovits-Bányai

37. MUTATION BREEDING BY ION BEAM IN LETTUCE (LACTUCA SATIVA L.) USING AN IN VITRO REGENERATION SYSTEM

S. Ohki | M. Hatashita

38. IN VITRO CONSERVATION OF REDWOOD (SEQUOIA SEMPERVIRENS) BY SLOW GROWTH STORAGE AND CRYOPRESERVATION

E.A. Ozudogru | E. Kirdok | E. Kaya | M. Capuana | C. Benelli

39. PRELIMINARY INVESTIGATION ON VOLATILE TERPENE PROFILES FROM IN VITRO SHOOT CULTURES OF THYMUS VULGARIS L. CLONES

E.A. Ozudogru | G. Cencetti | M. Michelozzi

40. EVALUATION OF GENETIC FIDELITY OF IN VITRO PROPAGATED APPLE (MALUS × DOMESTICA BORKH.) ROOTSTOCK MM 106 USING ISSR MARKERS

H. Pathak | V. Dhawan

41. INFLUENCE OF DIFFERENT CARBOHYDRATE SOURCES ON IN VITRO SHOOT PROLIFERATION OF APPLE (MALUS × DOMESTICA BORKH.) ROOTSTOCKS M 7 AND MM 111

H. Pathak | V. Dhawan

42. RECENT ADVANCES IN ANDROGENESIS INDUCTION OF CASSAVA (MANIHOT ESCULENTA CRANTZ)

43. SOLANUM PENNELLII LA716 AS A SOURCE OF GENES IMPROVING IN VITRO ORGANOGENESIS IN CULTIVATED TOMATO

F.N. Arikita | M.S. Azevedo | L.E.P. Peres | D.C. Scotton | A. Figueira

44. IN VITRO CULTURE REVEALS DIFFERENCES IN THE ORGAN RESPONSIBLE FOR CITRUS RESPONSES TO OSMOTIC OR SALT STRESS

45. EARLY IDENTIFICATION OF TOMATO GENOTYPES EXPRESSING MUTATIONS INVOLVED IN THE SYNTHESIS OF ANTHOCYANINS

E. Santangelo | M.E. Picarella | A. Mazzucato | G.P. Soressi

46. POTENTIALS OF CALLUS CULTURE AS A BREEDING TOOL FOR POLYPLOIDISATION OF FRAGARIA VESCA L.

I. Pinker | K. Olbricht | F. Pohlheim

47. SCREENING OF SOME DATE PALM CULTIVARS TO SALT STRESS IN VITRO

Y.M. Ibraheem | I. Pinker | M. Böhme | Z. Al-Hussin

48. THE EFFECT OF SODIUM CHLORIDE-STRESS ON ‘ZAGHLOOL’ DATE PALM SOMATIC EMBRYOGENESIS

Y.M. Ibraheem | I. Pinker | M. Böhme

49. THE EFFECT OF FLOWER BUD SIZE AND PRE-TREATMENT ON CALLUS INDUCTION AND PLANT REGENERATION IN ANTHER CULTURE OF PETUNIA ‘PURPLE WAVE’

M. Chaar | I. Pinker | P. Grieger | M. Böhme

50. SEED AND SEEDLING SURFACE-STERILIZATION FOR IN VITRO CULTURE OF TILLANDSIA GARDNERI (BROMELIACEAE)

A.C.R. Pinto | M.E.S.P. Demattê | S. Creste | J.C. Barbosa

51. IN VITRO TETRAPLOID INDUCTION IN TULIP (TULIPA GESNERIANA L.)

M. Podwyszyńska

52. GENETIC TRANSFORMATION OF FRAGARIA WITH CANDIDATE GENES INVOLVED IN DROUGHT STRESS

53. INHERITANCE FOR EARLINESS IN ORNAMENTAL PEPPERS (CAPSICUM ANNUUM)

54. IN VITRO MORPHOGENIC POTENTIAL OF TWO GENOTYPES OF COWPEA (VIGNA UNGUILATA L. WALP.)

M.M. do Rêgo | E.R. do Rêgo | G.F. Araujo | W.C. Otoni

55. ARTIFICIAL SEED PRODUCTION FROM ENCAPSULATED MICROSHOOTS OF CAULIFLOWER (BRASSICA OLERACEAE VAR. BOTRYTIS)

H.Z. Rihan | M. Al-Issawi | S. Burchett | M.P. Fuller

56. THE PRODUCTION OF CAULIFLOWER MICROSHOOTS USING CURD MERISTEMATIC TISSUES AND HYPOCOTYL-DERIVED CALLUS

H.Z. Rihan | M. Al Shamari | M.P. Fuller

57. APPLICATION OF A NEW METHOD TO OBTAIN CELL BIOMASS OF THEOBROMA CACAO SEEDS FOR METABOLITES PRODUCTION IN FLASKS AND BIOREACTOR CULTURES

L.F. Rojas | C. Florez | L. Atehortúa | A. Gil | J. Londoño

58. MATURATION OF OLIVE SOMATIC EMBRYOS

H. Benzekri | C. Sánchez-Romero

59. STUDIES ON IN VITRO REGENERATION FROM VARIOUS EXPLANTS OF A MATURE NEEM (AZADIRACHTA INDICA) TREE

60. IMPROVING MICROPROPAGATION PERFORMANCES IN HYDRANGEA SPP.: TEMPORARY IMMERSION SHOOT CULTURE AND INDUCTION OF MORPHOGENETIC EVENTS

M. Savona | E. Sacco | B. Ruffoni

61. PHYSIOLOGICAL RESPONSE TO NITROGEN DEFICIENCY STRESS OF IN VITRO GROWN POTATO GENOTYPES

A. Schum | G. Jansen

62. MICROPROPAGATION OF OLD PEAR CULTIVARS

J. Sedlak | F. Paprstein

63. INDUCTION OF MULTI-CELLULAR STRUCTURES IN ISOLATED MICROSPORES OF ROSES (ROSA HYBRID)

M. Oroojloo | M.E. Shariatpanahi

64. BIOCHEMICAL AND MORPHOLOGICAL CHANGES IN MICROPROPAGATED SHOOTS OF GISELA6^® (PRUNUS SPP.) ROOTSTOCK INOCULATED WITH TRICHODERMA HARZIANUM STRAIN T-22

A. Sofo | G. Tataranni | A. Scopa | B. Dichio | C. Xiloyannis

65. SOMATIC SEGREGATION: GENETIC EVIDENCES, CONSEQUENCES AND APPLICATIONS

66. A SCREEN FOR THE IDENTIFICATION OF MALE MEIOTIC MUTANTS BASED ON POLLEN SIZE

M.S. Tholath Lazar | N. De Storme | D. Geelen

67. POWER AND LIMITATIONS OF DICISTRONIC VECTOR SYSTEMS FOR PLANT TRANSFORMATIONS

L.A.I. Vaas | M. Marheine | E. Heine-Dobbernack | H.M. Schumacher | H. Kiesecker

68. VOLUMES OF APOPLASTIC WATER AND AIR IN HYPERHYDRIC LEAVES OF ARABIDOPSIS THALIANA SEEDLINGS

N. van den Dries | F.A. Krens | S. Gianní | G.J.M. de Klerk

69. EFFICACY OF RHIZOBACTERIA ON PLANT GROWTH PROMOTION AND DISEASE SUPPRESSION IN VITRO

70. EFFECT OF RED-, FAR-RED- AND BLUE-LIGHT-EMITTING DI-ODES ON IN VITRO GROWTH OF FICUS BENJAMINA

S. Werbrouck | H. Buyle | D. Geelen | M.C. Van Labeke

71. MANIPULATION OF PLOIDY FOR KIWIFRUIT BREEDING AND THE STUDY OF ACTINIDIA GENOMICS

Jin-Hu Wu

72. HIGH THROUGHPUT MARKER DEVELOPMENT AND APPLICATION IN HORTICULTURAL CROPS

M.J.M. Smulders | M. Vukosavljev | A. Shahin | W.E. van de Weg | P. Arens

73. DEVELOPMENT OF NEW VARIETAL TYPES BASED ON REJUVENATION BY SOMATIC EMBRYOGENESIS AND PROPAGATION BY CONVENTIONAL BUDDING OR MICROCUTTING IN HEVEA BRASILIENSIS

74. CHARACTERIZATION OF SOMATIC HYBRIDS SOLANUM BULBOCASTANUM + DIHAPLOID SOLANUM TUBEROSUM

75. IMMATURE SEED RESCUE AND ABSCISIC ACID QUANTIFICATION IN ROSA HYBRIDA L. SUGGEST EARLY AND TRANSIENT ENDODORMANCY

76. INVESTIGATIONS ON IN VITRO MULTIPLICATION AND ROOTING IN CAMELLIA JAPONICA L.

L. Seglie | M. Caser | A. Berruti | V. Scariot

77. AN IN VITRO BIOASSAY FOR THE EVALUATION OF COLD TREATMENT ON FLOWER BUD DORMANCY IN CAMELLIA

A. Berruti | A. Christiaens | M.C. Van Labeke | V. Scariot