Articles
A SCREEN FOR THE IDENTIFICATION OF MALE MEIOTIC MUTANTS BASED ON POLLEN SIZE
Article number
961_66
Pages
503 – 509
Language
English
Abstract
Meiosis is a specific cell division that produces four haploid gametes from diploid parental cells, in two rounds of chromosome segregation.
Formation of unreduced gametes and polyploidization are the main consequences caused by any defect through the progression of meiosis.
To date, a quite number of meiotic genes involved in diverse range of meiotic processes have been identified.
The main aim of the work was to identify new mutants showing unreduced gamete formation, due to abnormal meiosis.
We have screened a pool of EMS mutagenized Arabidopsis thaliana lines based on the presence of large pollen.
However, the fertility and ploidy of the mutants were also considered as main criteria for the screening of the mutant plants.
Here we describe mutants producing polyads, which clearly depicts a defect during meiosis.
Further research will be to determine the meiotic defect caused and to localize the responsible gene.
Formation of unreduced gametes and polyploidization are the main consequences caused by any defect through the progression of meiosis.
To date, a quite number of meiotic genes involved in diverse range of meiotic processes have been identified.
The main aim of the work was to identify new mutants showing unreduced gamete formation, due to abnormal meiosis.
We have screened a pool of EMS mutagenized Arabidopsis thaliana lines based on the presence of large pollen.
However, the fertility and ploidy of the mutants were also considered as main criteria for the screening of the mutant plants.
Here we describe mutants producing polyads, which clearly depicts a defect during meiosis.
Further research will be to determine the meiotic defect caused and to localize the responsible gene.
Authors
M.S. Tholath Lazar, N. De Storme, D. Geelen
Keywords
pollen, meiotic mutants, fertility, polyploids, polyads
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