Articles
DETERMINATION OF THE OPTIMUM MICROSPORE DEVELOPMENT STAGE AND OPTIMUM CULTURE MEDIUM IN ASPARAGUS (ASPARAGUS OFFICINALIS VAR. ALTILIS L.) FOR ANTHER CULTURE
Article number
961_17
Pages
153 – 157
Language
English
Abstract
Asparagus is a dioecious vegetable.
The main target of asparagus breeders is to obtain hybrids composed of exclusively male plants, since they are more productive than female ones.
Anther culture is a method to obtain haploids that can be doubled with colchicine treatment.
In order to determine the optimal microspore development stage for androgenesis, anthers were stained with ethidium bromide and examined under fluorescence microscope.
Anthers with microspores at the late uninucleate stage were cultured on Murashige and Skoog (MS) basal medium supplemented with three growth regulators at different concentrations (1 mg L-1 2.4 D + 0.1 mg L-1 BA + 0.5 mg L-1 NAA; 1 mg L-1 BA + 5 mg L-1 NAA; 0.5 mg L-1 BA + 1 mg L-1 NAA). The highest number of embryos (8.33%) and the number of plantlets (13.33%) were obtained on MS medium with 0.5 mg L-1 BA and 1 mg L-1 NAA.
The main target of asparagus breeders is to obtain hybrids composed of exclusively male plants, since they are more productive than female ones.
Anther culture is a method to obtain haploids that can be doubled with colchicine treatment.
In order to determine the optimal microspore development stage for androgenesis, anthers were stained with ethidium bromide and examined under fluorescence microscope.
Anthers with microspores at the late uninucleate stage were cultured on Murashige and Skoog (MS) basal medium supplemented with three growth regulators at different concentrations (1 mg L-1 2.4 D + 0.1 mg L-1 BA + 0.5 mg L-1 NAA; 1 mg L-1 BA + 5 mg L-1 NAA; 0.5 mg L-1 BA + 1 mg L-1 NAA). The highest number of embryos (8.33%) and the number of plantlets (13.33%) were obtained on MS medium with 0.5 mg L-1 BA and 1 mg L-1 NAA.
Authors
N. Ercan, F. Ayar Şensoy
Keywords
asparagus, anther culture, bud size, basal medium
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