RETROTRANSPOSITION EVENTS IN BARLEY CALLUS CULTURES

Gene transfer techniques offer an alternative to improve existing cultivars and to obtain new ones.
Establishment of tissue culture procedures is a prerequisite in order to obtain stable transformants.
However, plants regenerated from tissue culture may exhibit somaclonal variation.
These variations, generally undesired, include nucleotide mutations, gene activation/silencing, chromosome abnormalities.
Gene silencing and activation occur by DNA methylation and activation of transposable elements.
Movement of transposable elements also causes insertional polymorphisms in genome.
DNA polymorphisms in 15-day-old, 30-day-old calli and 4-day-old barley (Hordeum vulgare ‘Zafer-160’) seedling were investigated with Inter-Retrotransposon Amplified Polymorphism (IRAP), a retrotransposon-based marker system.
PCR primers designed from BARE-1 and Sukkula elements were combined and amplification products were resolved on non-denaturing polyacrylamide gels.
Different polymorphic bands were detected, although banding patterns were highly monomorphic among different tissues.
However, control (4-day-old seedling) tissue and 30-day-old callus gave more similar fingerprints.

VII International Symposium on In Vitro Culture and Horticultural Breeding

A. Temel, N. Gozukirmizi

retrotransposon, IRAP, BARE-1, Sukkula, polymorphism

https://doi.org/10.17660/ActaHortic.2012.961.20