Articles
THE EFFECT OF SODIUM CHLORIDE-STRESS ON ‘ZAGHLOOL’ DATE PALM SOMATIC EMBRYOGENESIS
Article number
961_48
Pages
367 – 373
Language
English
Abstract
This study was conducted to investigate the effect of salt stress on callus proliferation, somatic embryo formation and conversion of Zaghlool date palm in vitro.
For callus proliferation, 200 mg portions of date palm embryogenic callus were subjected to MS medium with 10 mg/L 2.4-D, 3 mg/L 2iP and 1.5 g/L activated charcoal supplied with different NaCl concentrations (0, 25, 75, 125, 175, 225 mM) for 3 subcultures.
The fresh weight was recorded in 4-week-intervals.
The dry weight and chloride content were evaluated at the end of the experiment.
The callus fresh and dry weight were enhanced by adding 25 mM NaCl to the proliferation medium; higher salt concentrations affected the callus growth negatively.
Callus growth ceased totally on medium with 225 mM NaCl.
Some somatic embryos were formed already on callus proliferation medium; the number of embryos was enhanced by adding 25 mM NaCl to the proliferation medium.
For somatic embryo induction, 200 mg portions of embryogenic callus were cultured on regeneration medium with 0.1 mg/L NAA and 1.5 g/L activated charcoal supported with the same afore mentioned NaCl concentrations.
The number of somatic embryos increased in the presence of 25 mM NaCl.
At 75 mM NaCl less somatic embryos were formed. 175 and 225 mM NaCl completely inhibited somatic embryo formation.
Somatic embryo conversion was negatively affected by increasing the salt concentration to 125 mM. Callus accumulated NaCl and there was high positive correlation with the salt concentration in the proliferation and regeneration media.
The chloride content was higher in cell grown on the proliferation medium than on regeneration medium.
For callus proliferation, 200 mg portions of date palm embryogenic callus were subjected to MS medium with 10 mg/L 2.4-D, 3 mg/L 2iP and 1.5 g/L activated charcoal supplied with different NaCl concentrations (0, 25, 75, 125, 175, 225 mM) for 3 subcultures.
The fresh weight was recorded in 4-week-intervals.
The dry weight and chloride content were evaluated at the end of the experiment.
The callus fresh and dry weight were enhanced by adding 25 mM NaCl to the proliferation medium; higher salt concentrations affected the callus growth negatively.
Callus growth ceased totally on medium with 225 mM NaCl.
Some somatic embryos were formed already on callus proliferation medium; the number of embryos was enhanced by adding 25 mM NaCl to the proliferation medium.
For somatic embryo induction, 200 mg portions of embryogenic callus were cultured on regeneration medium with 0.1 mg/L NAA and 1.5 g/L activated charcoal supported with the same afore mentioned NaCl concentrations.
The number of somatic embryos increased in the presence of 25 mM NaCl.
At 75 mM NaCl less somatic embryos were formed. 175 and 225 mM NaCl completely inhibited somatic embryo formation.
Somatic embryo conversion was negatively affected by increasing the salt concentration to 125 mM. Callus accumulated NaCl and there was high positive correlation with the salt concentration in the proliferation and regeneration media.
The chloride content was higher in cell grown on the proliferation medium than on regeneration medium.
Authors
Y.M. Ibraheem, I. Pinker, M. Böhme
Keywords
Phoenix dactylifera L., NaCl, in vitro screening, callus proliferation
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