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Articles

AN IMPROVED MICRO PROPAGATION SYSTEM FOR SUCCESSFUL TRANSFORMATION OF CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS)

Article number

961_19

Pages

167 – 174

Language

English

Abstract

Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems were used in a fractionation and sieving protocol to produce microshoots in in vitro culture.
This technique produced many thousands of small explants suitable for transfection studies.
Explants were successfully transformed following standard transfection with A. tumefaciens carrying a construct with Kanamycin^R, GUS and the Ascorbate Peroxidase (APX) gene.
Selection using Kanamycin produced normal plant phenotypic plantlets and these putative transformants were confirmed using GUS staining and PCR of the APX gene sequence.
The transfection conditions were optimised during this study and 105 Agrobacterium cells/5 plants/pot successfully produced 1 transformed plant/pot.

Publication

VII International Symposium on In Vitro Culture and Horticultural Breeding

Authors

F.G. Al-Swedi, S. Lane, M.P. Fuller

Keywords

cauliflower, micro propagation, Agrobacterium tumefaciens

Full text

https://doi.org/10.17660/ActaHortic.2012.961.19

Online Articles (77)

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J.W. Grosser | M. Calović | F.G. Gmitter Jr.

2. IN VITRO STORAGE AND CRYOPRESERVATION AS SUBSTANTIAL COMPLEMENTS IN CONCERTED ACTIONS TO BETTER MAINTAIN AND USE CROP GERMPLASM

E.R.J. Keller | B. Panis | F. Engelmann

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H. Lütken | E.B. Jensen | S.V. Wallström | R. Müller | B. Christensen

4. PRELIMINARY RESULTS ON THE USE OF MELIA AZEDARACH FRUIT EXTRACTS AS ANTIMICROBIAL AGENTS IN THE MICROPROPAGATION OF ZANTEDESCHIA

G. Marino | F. Gaggìa | B. Ruffoni

5. EXTENSIVE CITRUS TRIPLOID BREEDING PROGRAM BY 2X×4X SEXUAL HYBRIDIZATIONS

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7. CONTAMINATION DURING MICROPROPAGATION: ANALYSIS OF THE BACTERIAL LINES AND TREATMENT WITH SAGE EXTRACT

8. IN VITRO CONSERVATION OF ORNAMENTAL PLANTS BY SLOW GROWTH STORAGE

C. Benelli | E.A. Ozudogru | M. Lambardi | G. Dradi

9. OPTIMISATION OF SOMATIC HYBRIDISATION IN CICHORIUM SPECIES

D. Deryckere | T. Eeckhaut | J. Van Huylenbroeck | E. Van Bockstaele

10. ORNAMENTAL PLANT TISSUE CULTURE INDUSTRY IN FLANDERS (BELGIUM)

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11. A PRELIMINARY INVESTIGATION INTO HOW PLANT GROWTH PROMOTING RHIZOBACTERIA CAN INFLUENCE THE FREQUENCY OF GENE TRANSFER IN AGROBACTERIUM– AND TRANSBACTER^TM-MEDIATED TRANSFORMATION SYSTEMS

B. Doyle Prestwich | O. O'Herlihy

12. EXPRESSION OF REGENERATION COMPETENCE FOR ADVENTITIOUS BUDDING, ROOTING AND SOMATIC EMBRYOGENESIS COMPARING TEMPERATE FRUIT TREE SPECIES

PH. Druart | K. Ben Mahmoud | S. Richet | V. Kondakhova

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16. TOWARDS IN VITRO SELECTION FOR SALINITY TOLERANCE IN APRICOT CULTIVAR ‘CANINO’

A.S. El-sabagh | M.N. Barakat | E.A.-E. Genaidy

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N. Ercan | F. Ayar Şensoy

18. PLANT BIODIVERSITY AT HIGH ALTITUDE: IN VITRO PRESERVATION

P. Fasciani | G. Pirone | L. Pace

19. AN IMPROVED MICRO PROPAGATION SYSTEM FOR SUCCESSFUL TRANSFORMATION OF CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS)

F.G. Al-Swedi | S. Lane | M.P. Fuller

20. RETROTRANSPOSITION EVENTS IN BARLEY CALLUS CULTURES

A. Temel | N. Gozukirmizi

21. TOWARDS PHENOTYPING HEAT TOLERANCE IN GARDEN ROSES

O.F.C. Greyvenstein | T.W. Starman | H.B. Pemberton | G. Niu | D.H. Byrne

22. EFFECT OF GA₃ AND PACLOBUTRAZOL ON ADVENTITIOUS SHOOT REGENERATION OF TWO PELARGONIUM SP.

23. PRODUCTION OF TRANSGENIC APPLE (MALUS DOMESTICA BORKH.) FOR IMPROVEMENT OF FUNGAL RESISTANCE

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24. USE OF AFLP-MARKERS FOR ESTIMATION OF THE INBREEDING LEVEL IN HELLEBORUS ORIENTALIS

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B. Křižan | E. Ondrušiková | J. Moudrá | M. Pidra

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S. Ohki | M. Hatashita

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47. SCREENING OF SOME DATE PALM CULTIVARS TO SALT STRESS IN VITRO

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48. THE EFFECT OF SODIUM CHLORIDE-STRESS ON ‘ZAGHLOOL’ DATE PALM SOMATIC EMBRYOGENESIS

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49. THE EFFECT OF FLOWER BUD SIZE AND PRE-TREATMENT ON CALLUS INDUCTION AND PLANT REGENERATION IN ANTHER CULTURE OF PETUNIA ‘PURPLE WAVE’

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H.Z. Rihan | M. Al-Issawi | S. Burchett | M.P. Fuller

56. THE PRODUCTION OF CAULIFLOWER MICROSHOOTS USING CURD MERISTEMATIC TISSUES AND HYPOCOTYL-DERIVED CALLUS

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L.F. Rojas | C. Florez | L. Atehortúa | A. Gil | J. Londoño

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59. STUDIES ON IN VITRO REGENERATION FROM VARIOUS EXPLANTS OF A MATURE NEEM (AZADIRACHTA INDICA) TREE

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Jin-Hu Wu

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76. INVESTIGATIONS ON IN VITRO MULTIPLICATION AND ROOTING IN CAMELLIA JAPONICA L.

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77. AN IN VITRO BIOASSAY FOR THE EVALUATION OF COLD TREATMENT ON FLOWER BUD DORMANCY IN CAMELLIA

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