Articles
EFFECT OF GENOTYPE ON ORGANOGENESIS IN SIX GRAPE ROOTSTOCKS
Article number
961_27
Pages
225 – 229
Language
English
Abstract
The study on micropropagation of six grape rootstock genotypes namely Kober 5BB, Kober 125AA, Teleki 5C, SO4, Craciunel 2 and Amos was performed.
The aim of the study was to develop an optimized protocol to obtain large quantity of plant material.
Protocol is based on regeneration via organogenesis, considering that grape embryogenic calli are laborious to establish and the genotype of the regenerated plants can be altered.
Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks.
Subsequent cultivation on modified Murashige Skoog media with
1-naphtalene acetic acid and increased concentration of cytokinin was characterized by multiplication of cultures and formation of clusters with high multiplication capability.
Only genotype Amos presented low multiplication rate (1:3.2) and also callus production, which could be caused by its different parentage.
The average multiplication rates of genotypes (except Amos) varied from 1:8.7 to 1:14 in one month.
The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.
The aim of the study was to develop an optimized protocol to obtain large quantity of plant material.
Protocol is based on regeneration via organogenesis, considering that grape embryogenic calli are laborious to establish and the genotype of the regenerated plants can be altered.
Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks.
Subsequent cultivation on modified Murashige Skoog media with
1-naphtalene acetic acid and increased concentration of cytokinin was characterized by multiplication of cultures and formation of clusters with high multiplication capability.
Only genotype Amos presented low multiplication rate (1:3.2) and also callus production, which could be caused by its different parentage.
The average multiplication rates of genotypes (except Amos) varied from 1:8.7 to 1:14 in one month.
The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.
Authors
B. Křižan, E. Ondrušiková, J. Moudrá, M. Pidra
Keywords
grape, in vitro propagation, plant growth regulators, rootstocks, regeneration
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