GENETIC STABILITY IN MICRO-CLONES OF ‘WOOD-APPLE’ DERIVED FROM DIFFERENT PATHWAYS OF MICROPROPAGATION AS REVEALED BY RAPD AND ISSR MARKERS

Micropropagation of Feronia limonia, a medicinally important plant belonging to the family Rutaceae was achieved using two different pathways involving enhanced axillary branching and callus organogenesis.
Shoot cultures derived from them were sub-cultured up to ten passages and micro-clones were subjected to test of fidelity using RAPD and ISSRs markers.
Of 78 primers (53 RAPD + 25 ISSRs), 35 primers (27 RAPD + 8 ISSRs) produced clear, distinct and scorable bands with an average of 8.1 bands per primer.
Molecular analysis of the data revealed that micro-clones derived from mature node explants showed no variability while clones derived from cotyledonary node explants showed two percent variability.
Micro-propagules obtained from callus organogenesis pathway showed six percent variability.
Screening of micro-clones revealed that true-to-type plantlets could be obtained only through enhanced axillary branching using mature node segments.

VII International Symposium on In Vitro Culture and Horticultural Breeding

P. Joshi, S.D. Purohit

molecular markers, genetic fidelity, Feronia limonia, micropropagation, axillary branching, callus organogenesis, medicinal plant

https://doi.org/10.17660/ActaHortic.2012.961.26