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Articles

MOLECULAR MARKERS ASSOCIATED WITH COLD-HARDINESS IN CAMELLIA

Article number
1000_57
Pages
415 – 421
Language
English
Abstract
Sequence-characterized amplified region (SCAR) markers from expressed sequence tag-polymerase chain reaction (EST-PCR) and random amplified poly-morphic DNA (RAPD) markers were developed with the goal to separate cold hardy Camellia’s from non-cold hardy ones.
A total of 28 cold hardy and non-cold hardy Camellia genotypes were evaluated using 11 EST-PCR and 60 RAPD primers.
One EST-PCR primer pair based on a Camellia sinensis sequence (here referred to as CAME 13; forward, 5’ – CGGGCAGGTACCTTCTGATA – 3’ and reverse, 5’ – TGCAGAAGATGGAGATGCAG – 3’) and one RAPD primer (Operon A-12) appeared to have potential for discriminating between the cold hardy and non-cold hardy genotypes.
SCAR primer pairs were designed based on the sequence of the C. japonica bands amplified by the CAME 13 and Operon A-12 primers.
The EST-SCAR-19839 primer pair (forward, 5’ – CGGGCAGGTACCTTCTGATA – 3’; reverse, 5’ – CCTCAGCCGTAGTTGCATTT – 3’) proved useful for identifying non-cold hardy Camellia plants, whereas the fragment amplified by the RAPD-based SCAR primers appeared monomorphic.

Publication
VII International Symposium on New Floricultural Crops
Authors
Y.H. Joung, L.J. Rowland, J.Y. Kim, M.S. Roh
Keywords
DNA fingerprinting, sequence characterized amplified region (SCAR) markers, expressed sequence tags, polymerase chain reaction, random amplified polymorphic, DNA (RAPD) markers
Full text
https://doi.org/10.17660/ActaHortic.2013.1000.57
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