Articles
IN VITRO ESTABLISHMENT OF HELICONIA FROM FLORAL APEX
Article number
1000_79
Pages
553 – 558
Language
English
Abstract
The genus Heliconia, representative of the Heliconiaceae, is increasingly being included in flower-growing agribusiness.
This ornamental plant, cultivated exclusively in neotropical regions, presents inflorescences of various sizes and shapes, composed of bracts of different textures and color combinations.
The presence of innumerable endophytic microorganisms with different degrees of interaction with tissues is the factor that makes it difficult to standardize a technique to control their eradication in vegetable tissues, interfering in the successful micropropagation of this culture.
With the aim of evaluating the use of Heliconia inflorescences as an explant source for its in vitro establishment, three different stages of development were evaluated, with T1 being inflorescence with all the bracts closed, T2 with two open, T3 with four open, and at different concentrations of 6-benzylaminopurine (BAP) regulators, such as 0.0; 1.0; 3.0 and 5.0 mg L-1 and indolbutyric acid (IBA) such as 0.0 and 0.1 mg L-1 in MS (Murashige and Skoog) semi solid culture medium, (2.0 g L-1 phytagel), MS vitamins, sucrose (30 g L-1), in a total of 240 Heliconia rauliniana inflorescences.
At the intervals of the successive subcultures, the floral apices were evaluated with regard to the occurrence of contaminations, oxidation of tissues and bud development.
After six subcultures the inflorescence in stage T1 and the combination of 3.0 mg L-1 of BAP associated with 0.1 mg L-1 of IBA, presented satisfactory regeneration with the formation of viable budding and cuttings.
This ornamental plant, cultivated exclusively in neotropical regions, presents inflorescences of various sizes and shapes, composed of bracts of different textures and color combinations.
The presence of innumerable endophytic microorganisms with different degrees of interaction with tissues is the factor that makes it difficult to standardize a technique to control their eradication in vegetable tissues, interfering in the successful micropropagation of this culture.
With the aim of evaluating the use of Heliconia inflorescences as an explant source for its in vitro establishment, three different stages of development were evaluated, with T1 being inflorescence with all the bracts closed, T2 with two open, T3 with four open, and at different concentrations of 6-benzylaminopurine (BAP) regulators, such as 0.0; 1.0; 3.0 and 5.0 mg L-1 and indolbutyric acid (IBA) such as 0.0 and 0.1 mg L-1 in MS (Murashige and Skoog) semi solid culture medium, (2.0 g L-1 phytagel), MS vitamins, sucrose (30 g L-1), in a total of 240 Heliconia rauliniana inflorescences.
At the intervals of the successive subcultures, the floral apices were evaluated with regard to the occurrence of contaminations, oxidation of tissues and bud development.
After six subcultures the inflorescence in stage T1 and the combination of 3.0 mg L-1 of BAP associated with 0.1 mg L-1 of IBA, presented satisfactory regeneration with the formation of viable budding and cuttings.
Authors
H.V. Rodrigues, A.M.G. Gato, J.O. Pereira
Keywords
micropropagation, floriculture, inflorescence
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