Articles
MOLECULAR CYTOGENETIC CHARACTERIZATION OF WILD MEXICAN GEOPHYTES
Article number
1000_71
Pages
499 – 504
Language
English
Abstract
Mexico, with more than 25.000 different native plant germplasm, is known as the center of germplasm diversity, and many of these are utilized as ornamental plants worldwide.
Among different taxonomic families, some geophytic genera including Milla (Themidaceae), Sprekelia, Zephyranthes (Amarillydaceae) and Polianthes (Agavaceae) showed the beauty of their flowers and the genetic variability among their species.
The identification of individual chromosomes is a useful tool in breeding programs, from the ploidy determination to introgression of chromosomal segments in interspecific hybrids.
In the last decade, with the use of molecular cytogenetics, several species have been characterized.
However, there are still few probes that can be used for this purpose in characterizing plant species.
With the aim to identify individual chromosomes to assist in breeding program of selected ornamental species, we have generated several probes from repetitive DNA by cloning and sequencing.
The usefulness of the probes is shown by the identification of single chromosome through the use of molecular cytogenetics in different geophytes species with ornamental potential.
Among different taxonomic families, some geophytic genera including Milla (Themidaceae), Sprekelia, Zephyranthes (Amarillydaceae) and Polianthes (Agavaceae) showed the beauty of their flowers and the genetic variability among their species.
The identification of individual chromosomes is a useful tool in breeding programs, from the ploidy determination to introgression of chromosomal segments in interspecific hybrids.
In the last decade, with the use of molecular cytogenetics, several species have been characterized.
However, there are still few probes that can be used for this purpose in characterizing plant species.
With the aim to identify individual chromosomes to assist in breeding program of selected ornamental species, we have generated several probes from repetitive DNA by cloning and sequencing.
The usefulness of the probes is shown by the identification of single chromosome through the use of molecular cytogenetics in different geophytes species with ornamental potential.
Authors
E. Tapia-Campos, J.M. Rodriguez-Dominguez, E.E. Quiñones-Aguilar, P. Dupre, R. Barba-Gonzalez
Keywords
chromosome analysis, fluorescent in situ, hybridization, Milla, ornamental, Polianthes, Sprekelia, Zephyranthes
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