Articles
INFLUENCE OF ANTIBIOTICS, SEALING SYSTEM AND GENOTYPE ON SHOOT REGENERATION VIA ORGANOGENESIS IN PASSION FLOWERS
Article number
1000_78
Pages
445 – 452
Language
English
Abstract
In this work we evaluated the influence of three antibiotics (A) [Meropenem (MER), Cefotaxime (CEF) and Timentin (TIM)], three sealing systems-SS (PVPM, Petri dish, and PVC) and two genotypes (G) (Passiflora edulis FB 100 and P. cincinnata) on shoot regeneration from in vitro cultured leaf explants.
Explants were grown on MS medium supplemented with 2.0 mg.L-1 6-benzyladenine (BA) plus different concentrations of MER (0, 12.5 and 25.0 mg.L-1), CEF (0, 250 and 500 mg.
L-1) or TIM (0, 150 and 300 mg.L-1), for five days in the dark.
The flasks were sealed with three types of material (SS): polypropylene folding bag with microbial filter (ESPM), Petri dish with hypoallergenic tape (PDH), and a single layer of polyvinyl chlorine film (PVC). After explant inoculation and sealing of the flasks, these were transferred to a growth room at temperature 27±2°C. The flasks were transferred to a 16/8 h (light/dark) photoperiod, under irradiance of 36 μmol.m-2.s-1, for 30 days.
The experimental design was completely randomized in triple factorial scheme.
Each treatment was replicated four times, and each replicate consisted of ten leaf explants.
Shoot regeneration was evaluated 30 days after inoculation.
Data were subjected to variance analysis and means were compared by Tukeys test at 5% probability.
Percentage data were transformed in arcsin before analysis.
Significant differences were observed for G, SS, A, and double (G x SS and G x A) and triple (G x SS x A) interactions on the shoot regeneration.
In general, the best response with respect to G was obtained with P. edulis FB-100. For SS ESPM is more indicated, whereas TIM presented the better results among antibiotics evaluated.
Regarding to the triple interactions the best responses achieved were P. cincinnata x PDH x R (12.5 mg.L-1), and P. edulis x ESPM x TIM (150 mg.L-1). Based on these results we suggest the use of different protocols for the two species.
Explants were grown on MS medium supplemented with 2.0 mg.L-1 6-benzyladenine (BA) plus different concentrations of MER (0, 12.5 and 25.0 mg.L-1), CEF (0, 250 and 500 mg.
L-1) or TIM (0, 150 and 300 mg.L-1), for five days in the dark.
The flasks were sealed with three types of material (SS): polypropylene folding bag with microbial filter (ESPM), Petri dish with hypoallergenic tape (PDH), and a single layer of polyvinyl chlorine film (PVC). After explant inoculation and sealing of the flasks, these were transferred to a growth room at temperature 27±2°C. The flasks were transferred to a 16/8 h (light/dark) photoperiod, under irradiance of 36 μmol.m-2.s-1, for 30 days.
The experimental design was completely randomized in triple factorial scheme.
Each treatment was replicated four times, and each replicate consisted of ten leaf explants.
Shoot regeneration was evaluated 30 days after inoculation.
Data were subjected to variance analysis and means were compared by Tukeys test at 5% probability.
Percentage data were transformed in arcsin before analysis.
Significant differences were observed for G, SS, A, and double (G x SS and G x A) and triple (G x SS x A) interactions on the shoot regeneration.
In general, the best response with respect to G was obtained with P. edulis FB-100. For SS ESPM is more indicated, whereas TIM presented the better results among antibiotics evaluated.
Regarding to the triple interactions the best responses achieved were P. cincinnata x PDH x R (12.5 mg.L-1), and P. edulis x ESPM x TIM (150 mg.L-1). Based on these results we suggest the use of different protocols for the two species.
Authors
C.V. Silva, V.A.P. Loriato , W.C. Otoni , M.M. Rêgo , E.R. Rêgo
Keywords
Agrobacterium tumefaciens, gas exchange, genetic transformation, Passiflora
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