Articles
EFFECT OF NAPHTHALENE ACETIC ACID, 6-BENZYLAMINOPURINE AND THIDIAZURON ON THE IN VITRO PRODUCTION OF SHOOTS OF NEOREGELIA MARMORATA (BAKER) L.B. SMITH
Article number
1000_77
Pages
537 – 543
Language
English
Abstract
Bromeliads are widespread in Brazil, especially at the Atlantic Forest and their cultivation is also an economically viable alternative for floriculture.
The current investigation aimed to understand the in vitro growth and shooting of the bromeliad Neoregelia marmorata, as a tool for its preservation and commercial production.
Plantlets of N. marmorata obtained from in vitro germinated seeds, were cultured on MS media added with different concentrations of auxins and cytokinins.
The experimental design was entirely randomized, comprising of 12 treatments with NAA (Naphthalene Acetic Acid – 0; 1.34 or 2.69 µM) x BAP (6-benzylaminopurine – 0; 2.22; 4.44 or 8.87 µM) as well as 12 treatments with NAA (0; 1.34 or 2.69 µM) x TDZ (Thidiazuron – 0; 0.57; 1.14 or 2.27 µM). Increasing concentrations of BAP (2.22, 4.44 or 8.87 µM) in combination with 2.69 µM NAA produced the highest number of shoots.
TDZ did not show any positive response regarding the number of shoots, in fact probably that the concentrations used were phytotoxic.
NAA in conjunction with 2.22 or 4.44 µM BAP was also important in increasing the number of shoots, once again showing the importance of this auxin is shoot induction when associated with BAP.
The current investigation aimed to understand the in vitro growth and shooting of the bromeliad Neoregelia marmorata, as a tool for its preservation and commercial production.
Plantlets of N. marmorata obtained from in vitro germinated seeds, were cultured on MS media added with different concentrations of auxins and cytokinins.
The experimental design was entirely randomized, comprising of 12 treatments with NAA (Naphthalene Acetic Acid – 0; 1.34 or 2.69 µM) x BAP (6-benzylaminopurine – 0; 2.22; 4.44 or 8.87 µM) as well as 12 treatments with NAA (0; 1.34 or 2.69 µM) x TDZ (Thidiazuron – 0; 0.57; 1.14 or 2.27 µM). Increasing concentrations of BAP (2.22, 4.44 or 8.87 µM) in combination with 2.69 µM NAA produced the highest number of shoots.
TDZ did not show any positive response regarding the number of shoots, in fact probably that the concentrations used were phytotoxic.
NAA in conjunction with 2.22 or 4.44 µM BAP was also important in increasing the number of shoots, once again showing the importance of this auxin is shoot induction when associated with BAP.
Authors
A.R. Tavares, S. Kanashiro, R.F.G. Lima , J.A. Guariento, R.C.S. Ribeiro, M.M. Tanji
Keywords
Bromeliads, growth regulator, micropropagation, rooting, shooting
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