Articles
UTILIZING WILD SPECIES FOR MARKER-ASSISTED SELECTION OF CROP TIMING AND QUALITY TRAITS IN PETUNIA
Article number
1000_65
Pages
465 – 469
Language
English
Abstract
Marker-assisted selection (MAS) can improve the efficiency of breeding for complex traits.
However, MAS has rarely been utilized in floricultural crops due to the limited availability of genetic resources. Petunia ×hybrida is a valuable bedding plant for which some genomic resources have been developed.
Most Petunia species can be intercrossed to form hybrids, and wild relative species have been important sources of novel traits for P. ×hybrida.
Petunia axillaris and P. integrifolia are the progenitor species of P. ×hybrida. Two interspecific F2 populations, P. axillaris × P. exserta and P. integrifolia × P. axillaris, exhibited transgressive segregation for several crop timing and quality parameters, including leaf number below first flower, time to flower, leaf unfolding rate, branch number, flower bud number, and flower size, indicating their potential utility as sources of novel alleles for commercial petunia breeding.
To develop molecular markers for linkage mapping and quantitative trait locus analysis, 19,609 P. axillaris Plant GDB-assembled Unique Transcripts (PUTs) were searched for simple sequence repeats (SSRs), and 591 SSRs met the search criteria.
Of these, 132 sequences were polymorphic between P. axillaris and P. integrifolia, and 99 sequences were polymorphic between P. axillaris and P. exserta. Fifty-eight markers were polymorphic across all three parental species.
These markers will allow generating the genetic linkage maps for the two interspecific hybrid populations and initial identification of chromosomal regions controlling traits of interest.
However, MAS has rarely been utilized in floricultural crops due to the limited availability of genetic resources. Petunia ×hybrida is a valuable bedding plant for which some genomic resources have been developed.
Most Petunia species can be intercrossed to form hybrids, and wild relative species have been important sources of novel traits for P. ×hybrida.
Petunia axillaris and P. integrifolia are the progenitor species of P. ×hybrida. Two interspecific F2 populations, P. axillaris × P. exserta and P. integrifolia × P. axillaris, exhibited transgressive segregation for several crop timing and quality parameters, including leaf number below first flower, time to flower, leaf unfolding rate, branch number, flower bud number, and flower size, indicating their potential utility as sources of novel alleles for commercial petunia breeding.
To develop molecular markers for linkage mapping and quantitative trait locus analysis, 19,609 P. axillaris Plant GDB-assembled Unique Transcripts (PUTs) were searched for simple sequence repeats (SSRs), and 591 SSRs met the search criteria.
Of these, 132 sequences were polymorphic between P. axillaris and P. integrifolia, and 99 sequences were polymorphic between P. axillaris and P. exserta. Fifty-eight markers were polymorphic across all three parental species.
These markers will allow generating the genetic linkage maps for the two interspecific hybrid populations and initial identification of chromosomal regions controlling traits of interest.
Authors
J. Tychonievich, L. Wangchu, C. Barry, R.M. Warner
Keywords
breeding, floriculture, hybridization, interspecific, microsatellites, simple sequence repeats
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