Articles
Cryopreservation of in vitro grown Torenia using the cryo-plate vitrification method
Article number
1208_18
Pages
125 – 130
Language
English
Abstract
Torenia is a popular summer bedding plant that belongs to the family Linderniaceae. Recently, the number of vegetatively propagated cultivars has increased.
Cryopreservation is one of the most reliable techniques to keep mother plants for a long time.
Therefore, the aim of this study is to optimize the conditions for the cryo-plate vitrification method for Torenia. In this study, an in vitro culture of Torenia was used and the explants size, the duration and the temperature of the pre-culture, exposure time to PVS2 and mother plant age genotype differences were investigated.
Regrowth varied from 0 to 80% depending on the temperature conditions and genotypes.
For the Torenia hybrid ‘Summer Wave Blue’, the highest regrowth of 80% was obtained when small explants (0.2 mm) taken from 2- to 8-week-old mother plants were pre-cultured at 10°C for 2 d on 0.3 M sucrose MS medium and treated to PVS2 at room temperature for 10 min prior to LN. Other Torenia species showed lower regrowth than the hybrid.
Cryopreservation is one of the most reliable techniques to keep mother plants for a long time.
Therefore, the aim of this study is to optimize the conditions for the cryo-plate vitrification method for Torenia. In this study, an in vitro culture of Torenia was used and the explants size, the duration and the temperature of the pre-culture, exposure time to PVS2 and mother plant age genotype differences were investigated.
Regrowth varied from 0 to 80% depending on the temperature conditions and genotypes.
For the Torenia hybrid ‘Summer Wave Blue’, the highest regrowth of 80% was obtained when small explants (0.2 mm) taken from 2- to 8-week-old mother plants were pre-cultured at 10°C for 2 d on 0.3 M sucrose MS medium and treated to PVS2 at room temperature for 10 min prior to LN. Other Torenia species showed lower regrowth than the hybrid.
Publication
Authors
T. Laojunta, K. Tamiya, T. Narumi-Kawasaki, S. Fukai
Keywords
bedding plant, vegetatively propagated cultivars, PVS2, Linderniaceae, pre-culture
Groups involved
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Tropical and Subtropical Fruit and Nuts
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Commission Banana
- Division Horticulture for Development
- Division Precision Horticulture and Engineering
- Division Plant Genetic Resources, Breeding and Biotechnology
- Commission Cultivar Registration
- Division Plant-Environment Interactions in Field Systems
- Division Postharvest and Quality Assurance
- Division Greenhouse and Indoor Production Horticulture
- Division Landscape and Urban Horticulture
- Division Horticulture for Human Health
- Division Temperate Tree Nuts
- Commission Agroecology and Organic Farming Systems
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