Articles
A method for rapid separation of single cells from apple flesh
Article number
1208_9
Pages
61 – 68
Language
English
Abstract
As the basic units of organism, single living cells are the foundation of living activity and evolution.
The identification, separation and analysis of single living cells can be used to analyze deep level of heterogeneity and operation mechanism of a living system.
The study of fruit development is based on the extraction of active single cells.
By using the matured Fuji (Malus × domestica Borkh. ‘Fuji’) apples from the teaching and practicing base as the test material, this paper studies the impact of different enzyme liquid combinations and enzymolysis time on the single cell in apple flesh.
By using VBL to test cell wall, FDA to detect cell activity and fluo-4AM to determinate Ca2+ concentration, the results show that treating the apple flesh in 0.1% macerozyme solution for half an hour can provide a better condition for separating single cells.
The identification, separation and analysis of single living cells can be used to analyze deep level of heterogeneity and operation mechanism of a living system.
The study of fruit development is based on the extraction of active single cells.
By using the matured Fuji (Malus × domestica Borkh. ‘Fuji’) apples from the teaching and practicing base as the test material, this paper studies the impact of different enzyme liquid combinations and enzymolysis time on the single cell in apple flesh.
By using VBL to test cell wall, FDA to detect cell activity and fluo-4AM to determinate Ca2+ concentration, the results show that treating the apple flesh in 0.1% macerozyme solution for half an hour can provide a better condition for separating single cells.
Publication
Authors
Y.Q. Guan, H.Y. Qu
Keywords
apple, flesh, single cell, enzyme liquid concentration, enzymolysis time
Groups involved
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Tropical and Subtropical Fruit and Nuts
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Commission Banana
- Division Horticulture for Development
- Division Precision Horticulture and Engineering
- Division Plant Genetic Resources, Breeding and Biotechnology
- Commission Cultivar Registration
- Division Plant-Environment Interactions in Field Systems
- Division Postharvest and Quality Assurance
- Division Greenhouse and Indoor Production Horticulture
- Division Landscape and Urban Horticulture
- Division Horticulture for Human Health
- Division Temperate Tree Nuts
- Commission Agroecology and Organic Farming Systems
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