Comparative study of five different macroporous resins as separators and purifiers of apple polyphenols

The adsorption rules of five kinds of macroporous resins i.e., XDA-1, XDA-5, XAD-16, AB-8 and LH-20, either local or exotic, were used to study the isolation and purification of apple polyphenols in order to determine the appropriate solution to provide a technical basis for the effective measure on apple renewable resources.
The results indicated that different types of macroporous resin had a different maximum adsorption capacity (Max A) for apple polyphenol components, and among that XDA-1 had the highest Max A for total phenols with a measured value of 38.90 mg g^-1 and a model fitting value of 38.26 mg g^-1 (R²=0.9684), LH-20 had the highest Max A for flavanols with a measured value of 37.75 mg g^-1 and a model fitting value of 37.98 mg g^-1 (R²=0.9806). XAD-16 had the highest Max A for proanthocyanidins with a measured value of 29.60 mg g^-1 and a model fitting value of 30.05 mg g^-1 (R²=0.9766). The equilibrium time for apple polyphenol components to achieve Max A varied with different types of macroporous resin: it took 9 h for total phenols to reach 98.43% of Max A through XAD-16 while 10 h for flavanols to reach 93.18% of the Max A through AB-8 and proanthocyanidins to reach 94.86% of Max A through XDA-5. The desorption efficiency of apple polyphenol components also significantly varied according to the different concentrations of ethanol when XAD-16 reached adsorption equilibrium and the ratio for 50% ethanol to desorb total phenols was 95.05- 102.77% while for 70% ethanol to desorb both total phenols and proanthocyanidins, it went over 90%. For efficient purification of apple polyphenols while considering production cost, separation efficiency and product safety, XAD-16 macroporous resin with 50% ethanol was suggested as the best desorption solution.

II Asian Horticultural Congress

Qianru Zhang, Rong Yin, Zemin Duan, Xianping Wang

apple polyphenols, macroporous resin, adsorption capacity, desorption efficiency

https://doi.org/10.17660/ActaHortic.2018.1208.47