Articles
Effect of auxin concentration and pretreatment time on the efficiency of regeneration from leaf explants of plum rootstock
Article number
1260_11
Pages
59 – 64
Language
English
Abstract
Plant regeneration from a tissue culture system is often the most critical step in the success of various biotechnological techniques.
An efficient adventitious shoot regeneration protocol for ‘Elita’ plum rootstock ((Prunus pumila L. × P. salicina Lindl.) × (P. cerasifera Ehrh.)) was established.
The method was based on an auxin pretreatment of in vitro leaf explants applied before placement on a regeneration medium under standard culture conditions.
A high percentage of explants (up to 68.9%) showing shoot regeneration was obtained from leaves pre-cultured for 4 h in liquid MS medium supplemented with 2.5-5.0 mg L-1 indole-3-acetic acid.
The protocol presented here could facilitate the application of genetic engineering methods to produce mature plum trees with improved resistance to biotic and abiotic stresses.
An efficient adventitious shoot regeneration protocol for ‘Elita’ plum rootstock ((Prunus pumila L. × P. salicina Lindl.) × (P. cerasifera Ehrh.)) was established.
The method was based on an auxin pretreatment of in vitro leaf explants applied before placement on a regeneration medium under standard culture conditions.
A high percentage of explants (up to 68.9%) showing shoot regeneration was obtained from leaves pre-cultured for 4 h in liquid MS medium supplemented with 2.5-5.0 mg L-1 indole-3-acetic acid.
The protocol presented here could facilitate the application of genetic engineering methods to produce mature plum trees with improved resistance to biotic and abiotic stresses.
Authors
T.N. Sidorova, S.V. Dolgov
Keywords
somatic tissues, Prunus, stone fruits, adventitious regeneration
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