Articles
Propagation and field tests of ‘BN 4 Kr’ – a promising sharka resistant rootstock
Article number
1260_32
Pages
203 – 208
Language
English
Abstract
Different Mirobolan types are still largely used for Prunus sp. propagation.
The rootstock resistance has great practical importance in order to limit the spread of Plum pox virus (PPV), given that root suckers represent gateways for the virus.
Minoiu and Vlădianu (2001) had developed a mutant of Mirobolan, named BN 4 Kr at the Bistriţa Fruit Research and Development Station, which has been proven resistant to PPV (Minoiu et al., 1997, 1998). Other experiments have confirmed that aphid vectors are not able to transmit PPV to Mirobolan BN 4 Kr plants (Polák and Kominek, 2014). In order to check the potential of large-scale propagation of BN 4 Kr, green cuttings and in vitro propagation were studied.
Green cuttings propagation was assessed for three consecutive years using different rooting substrates: sand, perlite, and a 1:1 mix of sand and perlite.
The results revealed an average rooting between 75.3 and 85.9%, with the highest rooting percentage on the perlite variant.
In vitro propagation of BN 4 Kr rootstock was tested at Vitroplant Italia.
Best culture medium was Murashige and Skoog (1962) supplemented with 2.0 mg L-1 BA and with 0.01 mg L-1 IBA for stabilization, 1.0 mg L-1 BA and 0.01 mg L-1 IBA for multiplication, and 1.5 mg L-1 IBA for rooting.
In central Italy (Valdaso, Ancona Province) Polytechnic University of Marche (Ancona, Italy) tested the BN 4 Kr rootstocks in replanting conditions, using Myrobalan 29C as a control.
One-year-old trees of BN 4 Kr were planted in November 2007 and grafted with Fortune plum in February 2009. The production and fruit quality were recorded from 2011-2015. The Fortune on BN 4 Kr rootstock showed a production similar to Myrobalan 29C but with lower vigour and slightly bigger fruit size.
Likely BN 4 Kr rootstock induced a lower incidence of phytoplasma symptoms (ESFY), which are a growing problem in plum production.
The resistance to PPV correlated with the high potential of vegetative propagation, recommending BN 4 Kr as a promising Mirobolan rootstock for sharka containment.
The exclusive license for propagation and commercialization of BN 4 Kr was awarded to Vitroplant Italia, starting with 2016.
The rootstock resistance has great practical importance in order to limit the spread of Plum pox virus (PPV), given that root suckers represent gateways for the virus.
Minoiu and Vlădianu (2001) had developed a mutant of Mirobolan, named BN 4 Kr at the Bistriţa Fruit Research and Development Station, which has been proven resistant to PPV (Minoiu et al., 1997, 1998). Other experiments have confirmed that aphid vectors are not able to transmit PPV to Mirobolan BN 4 Kr plants (Polák and Kominek, 2014). In order to check the potential of large-scale propagation of BN 4 Kr, green cuttings and in vitro propagation were studied.
Green cuttings propagation was assessed for three consecutive years using different rooting substrates: sand, perlite, and a 1:1 mix of sand and perlite.
The results revealed an average rooting between 75.3 and 85.9%, with the highest rooting percentage on the perlite variant.
In vitro propagation of BN 4 Kr rootstock was tested at Vitroplant Italia.
Best culture medium was Murashige and Skoog (1962) supplemented with 2.0 mg L-1 BA and with 0.01 mg L-1 IBA for stabilization, 1.0 mg L-1 BA and 0.01 mg L-1 IBA for multiplication, and 1.5 mg L-1 IBA for rooting.
In central Italy (Valdaso, Ancona Province) Polytechnic University of Marche (Ancona, Italy) tested the BN 4 Kr rootstocks in replanting conditions, using Myrobalan 29C as a control.
One-year-old trees of BN 4 Kr were planted in November 2007 and grafted with Fortune plum in February 2009. The production and fruit quality were recorded from 2011-2015. The Fortune on BN 4 Kr rootstock showed a production similar to Myrobalan 29C but with lower vigour and slightly bigger fruit size.
Likely BN 4 Kr rootstock induced a lower incidence of phytoplasma symptoms (ESFY), which are a growing problem in plum production.
The resistance to PPV correlated with the high potential of vegetative propagation, recommending BN 4 Kr as a promising Mirobolan rootstock for sharka containment.
The exclusive license for propagation and commercialization of BN 4 Kr was awarded to Vitroplant Italia, starting with 2016.
Authors
F. Stănică, I. Zagrai, A. Feștilă, G. Zuccherelli, K. Zuccherelli, D. Neri, G. Murri, F. Massetani, L. Zagrai, I. Platon
Keywords
Prunus cerasifera, ‘Mirobolan’, Plum pox virus, cutting, in vitro
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