Articles
Development of a platform for the biotechnological appropiation of native plants of the Mexican Bajio region
Article number
1359_16
Pages
139 – 142
Language
English
Abstract
In the Mexican state of Guanajuato and the Bajío region, a large number of native plants with ecological, medical and industrial potential have been identified, these plants have been linked to the traditions and customs of the native population, but they have not received more extensive attention for their potential usefulness.
Tissue culture associated with genomic tools can help the development of systems that allow a rational use of these plants.
The objective of this work was to develop a biotechnological platform for the production of metabolites of commercial interest in Tagetes erecta (marigold) and Heliopsis longipes (aztec root) in a controlled way.
In the initial stage of the project, the in vitro cultures of these two plants were established, in vitro root and callus cultures were set up successfully using different concentrations of auxins and cytokinins.
Later, with molecular analysis identified the microRNAs and mRNAs associated with the synthesis of secondary metabolites.
The miRNAs whose expression was specific in roots and calli (miR156, miR164 and miR171) were selected, their expression in the in vitro culture systems and their mRNA targets were analyzed and associated to a specific metabolic pathways, (taxanes and flavonoids). The production of secondary metabolites in the cultures was determined by TLC and HPLC and associated to the molecular analysis; in order to establish a metabolite induction system under controlled conditions.
Tissue culture associated with genomic tools can help the development of systems that allow a rational use of these plants.
The objective of this work was to develop a biotechnological platform for the production of metabolites of commercial interest in Tagetes erecta (marigold) and Heliopsis longipes (aztec root) in a controlled way.
In the initial stage of the project, the in vitro cultures of these two plants were established, in vitro root and callus cultures were set up successfully using different concentrations of auxins and cytokinins.
Later, with molecular analysis identified the microRNAs and mRNAs associated with the synthesis of secondary metabolites.
The miRNAs whose expression was specific in roots and calli (miR156, miR164 and miR171) were selected, their expression in the in vitro culture systems and their mRNA targets were analyzed and associated to a specific metabolic pathways, (taxanes and flavonoids). The production of secondary metabolites in the cultures was determined by TLC and HPLC and associated to the molecular analysis; in order to establish a metabolite induction system under controlled conditions.
Authors
F. Olvera-Martínez, S. Junquera-Martínez, D. Aceves-Monreal, B. Bañuelos-Hernández, F. Albarrán-Tamayo, P.L. López de Alba, A. Cruz-Hernández
Keywords
Cempaxuchitl, chilcuague, in vitro culture, Heliopsis longipes, miRNAs, Tagetes erecta
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