Articles
Optimization of fed-batch culture for polyphenol production from Theobroma cacao cell culture
Article number
1359_37
Pages
281 – 288
Language
English
Abstract
Polyphenols, a group of secondary metabolites produced by cacao beans (Theobroma cacao) are known for their antioxidant effects and benefits to human health.
The increasing demand to find alternative methods to produce them at a commercial scale have focused on cell cultures and, more specifically, on batch culture.
However, fed-batch culture offers a better approach to produce them more efficiently.
To date no research has been published on the evaluation of fed-batch for polyphenol production in T. cacao cell cultures.
The aim of this study was to evaluate the effect of fed-batch culture in 7.5-L stirred tank bioreactor (STB), to improve cell growth and polyphenol production (TPC). A standard batch culture was performed in a 7.5-L STB to obtain growth kinetics, TPC and nutrient consumption.
Then, fed-batch culture was designed to supply depleted nutrients at specific times of culture.
The feed mediums were composed of macronutrients (M1), micronutrients (M2) and a combination of both (M3). The feed rates were 0.18, 0.25 and 0.36 L day-1. An improvement in the culture productivities was found.
The highest cell growth and TPC were obtained with the M2 medium and a feed rate of 0.18 L day-1 (1.01 g L day-1 and 19.07 mg GAE g‑1, respectively). These results support the evidence that micronutrients are essential for macronutrients assimilation and are early consumed in the culture.
On the other hand, TPC was significantly increased by fed-batch culture when compared to batch and cocoa beans of same variety of study cultured under field conditions.
The current study represents a novel investigation in Theobroma cacao cell cultures at a laboratory scale.
Consequently, the fed-batch culture allowed us to develop an alternative process that can be scaled-up to a plant pilot and later to an industrial scale.
The increasing demand to find alternative methods to produce them at a commercial scale have focused on cell cultures and, more specifically, on batch culture.
However, fed-batch culture offers a better approach to produce them more efficiently.
To date no research has been published on the evaluation of fed-batch for polyphenol production in T. cacao cell cultures.
The aim of this study was to evaluate the effect of fed-batch culture in 7.5-L stirred tank bioreactor (STB), to improve cell growth and polyphenol production (TPC). A standard batch culture was performed in a 7.5-L STB to obtain growth kinetics, TPC and nutrient consumption.
Then, fed-batch culture was designed to supply depleted nutrients at specific times of culture.
The feed mediums were composed of macronutrients (M1), micronutrients (M2) and a combination of both (M3). The feed rates were 0.18, 0.25 and 0.36 L day-1. An improvement in the culture productivities was found.
The highest cell growth and TPC were obtained with the M2 medium and a feed rate of 0.18 L day-1 (1.01 g L day-1 and 19.07 mg GAE g‑1, respectively). These results support the evidence that micronutrients are essential for macronutrients assimilation and are early consumed in the culture.
On the other hand, TPC was significantly increased by fed-batch culture when compared to batch and cocoa beans of same variety of study cultured under field conditions.
The current study represents a novel investigation in Theobroma cacao cell cultures at a laboratory scale.
Consequently, the fed-batch culture allowed us to develop an alternative process that can be scaled-up to a plant pilot and later to an industrial scale.
Authors
M.I. Restrepo, L. Atehortúa, L.F. Rojas
Keywords
Theobroma cacao, batch culture, fed-batch culture, macronutrients, micronutrients, polyphenols
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