Articles
First accounts of Zantedeschia sp. Dasheen and Konjac mosaic virus detection in Latvia
Article number
1359_21
Pages
173 – 178
Language
English
Abstract
Historically, in Latvia, calla lily (Zantedeschia sp.) is a decorative plant that is grown in greenhouses.
Around the years 2012-2013, local populations started to suffer from plant damage and crippled growth; plants lost their decorative properties.
The time coincided with new Zantedeschia cultivars being brought to Latvia from nurseries in the Netherlands and Germany.
No further measures were taken except the elimination of damaged plants.
Unfortunately, that wasnRSQUOt enough and symptoms spread.
Urgent measures were needed.
Plant micropropagation using meristem cultures was chosen as the pathogen elimination method.
For multiplication stage MS medium containing 2.5 mg L‑1 BAP and 2 mg L‑1 IAA was used.
A half-strength MS medium containing 1 mg L‑1 NAA was used for root growth initiation.
After acclimatization, infected plants were observed and virus detection was carried out with molecular methods.
For RNS extraction Thermo Scientific GeneJET Plant RNA Purification Kit was used, then reverse transcription Applied Biosystems High-Capacity cDNA Reverse Transcription Kit, PCR reaction using Solis BioDyneHOT FIREPol® Blend Master Mix.
For visualization 1.5% Agarose gel was used.
There are at least 16 viruses in the world known to affect calla lilies.
Two viruses DsMV (Dasheen mosaic virus) and KoMV (Konjac mosaic virus) were detected both in greenhouse calla lily and in vitro plantlets.
Although DsMV is a wildly spread calla lily virus it hasnt been previously reported in Latvia.
KoMV is less common calla lily virus.
It was previously reported in Japan, Korea, Taiwan, China, the Netherlands, New Zealand, Germany, Brazil, and for the first time now in Latvia.
Around the years 2012-2013, local populations started to suffer from plant damage and crippled growth; plants lost their decorative properties.
The time coincided with new Zantedeschia cultivars being brought to Latvia from nurseries in the Netherlands and Germany.
No further measures were taken except the elimination of damaged plants.
Unfortunately, that wasnRSQUOt enough and symptoms spread.
Urgent measures were needed.
Plant micropropagation using meristem cultures was chosen as the pathogen elimination method.
For multiplication stage MS medium containing 2.5 mg L‑1 BAP and 2 mg L‑1 IAA was used.
A half-strength MS medium containing 1 mg L‑1 NAA was used for root growth initiation.
After acclimatization, infected plants were observed and virus detection was carried out with molecular methods.
For RNS extraction Thermo Scientific GeneJET Plant RNA Purification Kit was used, then reverse transcription Applied Biosystems High-Capacity cDNA Reverse Transcription Kit, PCR reaction using Solis BioDyneHOT FIREPol® Blend Master Mix.
For visualization 1.5% Agarose gel was used.
There are at least 16 viruses in the world known to affect calla lilies.
Two viruses DsMV (Dasheen mosaic virus) and KoMV (Konjac mosaic virus) were detected both in greenhouse calla lily and in vitro plantlets.
Although DsMV is a wildly spread calla lily virus it hasnt been previously reported in Latvia.
KoMV is less common calla lily virus.
It was previously reported in Japan, Korea, Taiwan, China, the Netherlands, New Zealand, Germany, Brazil, and for the first time now in Latvia.
Authors
L. Purmale, A. Korica, R. Joffe
Keywords
calla lily, micropropagation, DsMV, KoMV, PCR
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