Articles
Tamarillo cell suspension-based bioreactors optimization of a novel system for elicited enzymatic production
Article number
1359_29
Pages
223 – 232
Language
English
Abstract
Plant molecular farming is the production of important metabolites in plants, with several advantages over other systems when considering their safety and potential for large-scale and low-cost production.
Plant cell suspension (PCS) cultures are regarded as one of the most suitable molecular farming platforms for plant-based production of valuable materials.
In this context, the primary goal of this project was developing a novel plant-based bioreactor system to produce bioactive compounds with applicability in the agri-food industry.
Based on effective protocols for tamarillo (Solanum betaceum Cav.) micropropagation and PCS cultures establishment from induced calli lines, cell growth has been optimized and characterized and stress-related intracellularly/extracellular protein profiles under in vitro controlled conditions characterized.
These assays showed that tamarillo PCS secrete a wide range of enzymes, namely proteases and glycoside hydrolases, commonly associated with inhibiting pathogen-derived cell wall degrading enzymes.
By using different biotic elicitors (e.g., chitosan and yeast extract), enzyme production was enhanced, particularly by chitosan (0.1 g L‑1), resulting in elicited extracts showing higher glycosidase, alkaline phosphatase, and protease activities.
Further proteomic analysis is being conducted to identify differentially expressed proteins between treatments and the effectiveness of the obtained extracts assayed on bioactivity tests.
The results obtained are good indicators of the potential of tamarillo cell suspension cultures as a novel system for safe and low-cost production of high-demand metabolites.
Plant cell suspension (PCS) cultures are regarded as one of the most suitable molecular farming platforms for plant-based production of valuable materials.
In this context, the primary goal of this project was developing a novel plant-based bioreactor system to produce bioactive compounds with applicability in the agri-food industry.
Based on effective protocols for tamarillo (Solanum betaceum Cav.) micropropagation and PCS cultures establishment from induced calli lines, cell growth has been optimized and characterized and stress-related intracellularly/extracellular protein profiles under in vitro controlled conditions characterized.
These assays showed that tamarillo PCS secrete a wide range of enzymes, namely proteases and glycoside hydrolases, commonly associated with inhibiting pathogen-derived cell wall degrading enzymes.
By using different biotic elicitors (e.g., chitosan and yeast extract), enzyme production was enhanced, particularly by chitosan (0.1 g L‑1), resulting in elicited extracts showing higher glycosidase, alkaline phosphatase, and protease activities.
Further proteomic analysis is being conducted to identify differentially expressed proteins between treatments and the effectiveness of the obtained extracts assayed on bioactivity tests.
The results obtained are good indicators of the potential of tamarillo cell suspension cultures as a novel system for safe and low-cost production of high-demand metabolites.
Authors
B. Casimiro, I. Lopes, M. Correia, J. Canhoto, L.F. Rojas, P. Veríssimo, S. Correia
Keywords
cell suspension cultures, molecular farming, plant-based products, scale-up production, Solanum betaceum
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