Articles
Ethylene concentration is influenced by polyamine addition during somatic organogenesis in lemon (Citrus limon)
Article number
1359_35
Pages
269 – 274
Language
English
Abstract
In many lemon cultivars, somatic organogenesis can be influenced by ethylene production.
Moreover, ethylene biosynthesis is known to be associated with polyamines.
Therefore, this study evaluated the effect of polyamines on somatic organogenesis and ethylene production of lemons.
To analyze this effect, three polyamines (putrescine, spermidine and spermine) were added at four different concentrations to Fino 49 and Verna 51 regenerating culture media.
Nodal segments deprived from pre-existing buds of these two cultivars were cultured in those media to evaluate their effect.
The result was that addition of polyamine at low concentrations could increase regeneration percentage and regeneration rate in Verna 51. Media containing 0.3 mM spermidine produced the best result: regeneration percentage increased by 52%, and the regeneration rate by 72%, compared to control conditions.
In contrast, the addition of polyamine had a negative effect on the regeneration of Fino 49, and the regeneration rate and regeneration percentage decreased at each concentration of polyamine.
No correlation was found between ethylene production and regeneration.
All concentrations of putrescine induced a marked decrease in ethylene levels, while ethylene detection decreased only for spermidine and spermine at the highest concentrations.
Moreover, ethylene biosynthesis is known to be associated with polyamines.
Therefore, this study evaluated the effect of polyamines on somatic organogenesis and ethylene production of lemons.
To analyze this effect, three polyamines (putrescine, spermidine and spermine) were added at four different concentrations to Fino 49 and Verna 51 regenerating culture media.
Nodal segments deprived from pre-existing buds of these two cultivars were cultured in those media to evaluate their effect.
The result was that addition of polyamine at low concentrations could increase regeneration percentage and regeneration rate in Verna 51. Media containing 0.3 mM spermidine produced the best result: regeneration percentage increased by 52%, and the regeneration rate by 72%, compared to control conditions.
In contrast, the addition of polyamine had a negative effect on the regeneration of Fino 49, and the regeneration rate and regeneration percentage decreased at each concentration of polyamine.
No correlation was found between ethylene production and regeneration.
All concentrations of putrescine induced a marked decrease in ethylene levels, while ethylene detection decreased only for spermidine and spermine at the highest concentrations.
Authors
V. Celdrán-Sánchez, M. Pérez-Jiménez, F. Córdoba, O. Pérez-Tornero
Keywords
adventitious shoot regeneration, citrus lemon, spermidine, spermine, putrescine
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