Reducing post-rooting dormancy in juneberry (Amelanchier alnifolia) using plant growth regulators and temperature treatments

A ten-week study was conducted to evaluate post-rooting dormancy of micropropagated juneberry.
Temperatures of 6 and 25°C were main plots, while ‘Thiessen’ and ‘Northline’ cultivars (pre-rooted or non-pre-rooted), and nine plant growth regulator (PGR) treatments were sub-plots.
Significant two-way and three-way interactions indicated the complexity of post-rooting dormancy and the difficulty when trying to overcome this dormancy.
Pre-rooted plantlets treated with 750 mg L^‑1 gibberellic acid (GA₄₊₇), 100 mg L^‑1 benzyladenine (BA) or 250 mg L^‑1 GA₄₊₇ + 100 mg L^‑1 BA treatments recorded the greatest leaf number. ‘Thiessen’ pre-rooted plantlets grown at 25°C also had the greatest leaf number.
Application of 250, 500 and 750 mg L^‑1 GA₄₊₇ for pre-rooted plantlets resulted in the tallest plants.
Pre-rooted juneberry plantlets given 100, 200, 300 mg L^‑1 BA and 250 mg L^‑1 GA₄₊₇ + 100 mg L^‑1 BA had the greatest branching along with ‘Thiessen’ plantlets grown at 25°C. Pre-rooted ‘Thiessen’ plantlets had the most biomass as fresh weight and dry weight and produced the greatest root volume.

XXXI International Horticultural Congress (IHC2022): International Symposium on In Vitro Technology and Micropropagated Plants

N.K. Ardayfio, W. Dai, H. Hatterman-Valenti

benzyladenine, cytokinin, gibberellic acid, juneberry, saskatoon berry, tissue culture

https://doi.org/10.17660/ActaHortic.2023.1359.5