Articles
Improved micropropagation of Prunus spp. rootstocks using temporary immersion systems
Article number
1359_32
Pages
249 – 254
Language
English
Abstract
In vitro conventional micropropagation using different types of culture recipients with semi-solid media is a very effective but time-consuming approach.
For large-scale propagation this increases the price of the propagules and reduces cloning rates.
Temporary immersion systems (TIS), in which explants are periodically immersed in liquid medium, have emerged as a solution to improve propagation effectiveness.
The contact between the explants and the culture medium occurs in short periods of time, being sufficient to an efficient nutrient and PGRs uptake.
Production upscaling is particularly important for cultures with a big market demand, including fruit trees propagated for grafting purposes.
Rootstocks have a very important role in scion performance including tolerance to pests and diseases and abiotic stresses.
In Portugal, Prunus spp. fruit crops, including almond and peach, are mainly grafted onto GF677 and GXN15 rootstocks (both Prunus persica × P. dulcis hybrids) because of their drought tolerance, adaptability to poor soils, and bacteria tolerance.
The aim of this work was to establish GF677 and GXN15 in vitro, optimize culture conditions, and define the best strategy to upscale propagation using SETIS® bioreactors.
Three MS-based culture media (M1: 1 mg L‑1 indole-3-butyric acid (IBA) 0.06 mg L‑1 6-benzylaminopurine (BAP); 0.10 mg L‑1 gibberellic acid (GA3); M2: 0.8 mg L‑1 BAP; 50 mg L‑1 citric acid; and M3: 0.5 mg L‑1 BAP; 50 mg L‑1 citric acid) were tested for rootstock establishment. GF677 was successfully established after three multiplication cycles.
M2 (77.6% multiplication rates) was chosen to evaluate multiplication on different containers: glass (56.0%) versus plastic (70.1%) in order to predict explants behavior in the SETIS bioreactors.
Shoots micropropagated in plastic containers with M2 medium were used for upscaling production in the bioreactors.
TIS parameters were defined (immersion time, PGRs concentration) and tested.
Preliminary results showed an increased hyperhydricity when higher BAP concentrations were tested.
Proliferated shoots are being assayed for ex vitro rooting and micrografting purposes.
For large-scale propagation this increases the price of the propagules and reduces cloning rates.
Temporary immersion systems (TIS), in which explants are periodically immersed in liquid medium, have emerged as a solution to improve propagation effectiveness.
The contact between the explants and the culture medium occurs in short periods of time, being sufficient to an efficient nutrient and PGRs uptake.
Production upscaling is particularly important for cultures with a big market demand, including fruit trees propagated for grafting purposes.
Rootstocks have a very important role in scion performance including tolerance to pests and diseases and abiotic stresses.
In Portugal, Prunus spp. fruit crops, including almond and peach, are mainly grafted onto GF677 and GXN15 rootstocks (both Prunus persica × P. dulcis hybrids) because of their drought tolerance, adaptability to poor soils, and bacteria tolerance.
The aim of this work was to establish GF677 and GXN15 in vitro, optimize culture conditions, and define the best strategy to upscale propagation using SETIS® bioreactors.
Three MS-based culture media (M1: 1 mg L‑1 indole-3-butyric acid (IBA) 0.06 mg L‑1 6-benzylaminopurine (BAP); 0.10 mg L‑1 gibberellic acid (GA3); M2: 0.8 mg L‑1 BAP; 50 mg L‑1 citric acid; and M3: 0.5 mg L‑1 BAP; 50 mg L‑1 citric acid) were tested for rootstock establishment. GF677 was successfully established after three multiplication cycles.
M2 (77.6% multiplication rates) was chosen to evaluate multiplication on different containers: glass (56.0%) versus plastic (70.1%) in order to predict explants behavior in the SETIS bioreactors.
Shoots micropropagated in plastic containers with M2 medium were used for upscaling production in the bioreactors.
TIS parameters were defined (immersion time, PGRs concentration) and tested.
Preliminary results showed an increased hyperhydricity when higher BAP concentrations were tested.
Proliferated shoots are being assayed for ex vitro rooting and micrografting purposes.
Authors
T. Lopes, M. Correia, A. Pedrosa, E. Baltazar, J. Canhoto, S. Correia
Keywords
GF677, micro-rootstocks, plant bioreactors, SETIS®, up-scaled production
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