Articles
Selection of markers for the fingerprinting of commercial and newly developed rootstocks
Article number
1448_25
Pages
203 – 210
Language
English
Abstract
Most commercial rootstock cultivars are Poncirus trifoliata hybrids that exhibit similar morphological traits, at least at the seedling stage.
Phenotypic markers/traits, such as leaf morphology and size and growth habit, do not always allow for the unambiguous identification of specific rootstock cultivars, especially if they were generated using the same parents.
Moreover, commercial rootstocks propagated by apomictic seeds can produce variable numbers of off-type (zygotic) seedlings, which in most cases can be eliminated by visual screening; in other cases, they have a similar morphological appearance to the nucellar ones.
In the present work, we analysed a set of INDELs and SNP markers using high-resolution melting (HRM) analysis.
The analysis was conducted on commercial rootstocks (Carrizo and C35 citranges; Swingle citrumelo; P. trifoliata Rubidoux; P. trifoliata Serra; C54, C57, and C22 citrandarins), on 4 Citrus latipes × P. trifoliata hybrids (F6P12, F5P12, F2P12, F6P13) obtained at CREA, and on a new CREA citrange (V×P). Other citrus accessions, such as pummelos, sweet oranges, C. micrantha, Mexican lime, ‘Sunki’ and ‘Cleopatra’ mandarins, and ‘Alemow’, were used as controls.
Sixteen loci were tested using HRM, of which 10 showed profiles that were easy to interpret.
The markers provided a total of 57 polymorphic curves.
The loci U15703 and Ac7a provided the lowest number of alleles, while loci Ac8a and ID55 showed the highest ones.
The 10 loci allowed the unambiguous discrimination of all accessions.
These markers were also useful in determining the proportion of nucellar and zygotic seedlings for the new CREA rootstocks and helped to identify off-types.
The HRM analysis was simple, fast, and cost-effective and allowed discrimination among rootstocks showing very similar morphological characteristics.
Phenotypic markers/traits, such as leaf morphology and size and growth habit, do not always allow for the unambiguous identification of specific rootstock cultivars, especially if they were generated using the same parents.
Moreover, commercial rootstocks propagated by apomictic seeds can produce variable numbers of off-type (zygotic) seedlings, which in most cases can be eliminated by visual screening; in other cases, they have a similar morphological appearance to the nucellar ones.
In the present work, we analysed a set of INDELs and SNP markers using high-resolution melting (HRM) analysis.
The analysis was conducted on commercial rootstocks (Carrizo and C35 citranges; Swingle citrumelo; P. trifoliata Rubidoux; P. trifoliata Serra; C54, C57, and C22 citrandarins), on 4 Citrus latipes × P. trifoliata hybrids (F6P12, F5P12, F2P12, F6P13) obtained at CREA, and on a new CREA citrange (V×P). Other citrus accessions, such as pummelos, sweet oranges, C. micrantha, Mexican lime, ‘Sunki’ and ‘Cleopatra’ mandarins, and ‘Alemow’, were used as controls.
Sixteen loci were tested using HRM, of which 10 showed profiles that were easy to interpret.
The markers provided a total of 57 polymorphic curves.
The loci U15703 and Ac7a provided the lowest number of alleles, while loci Ac8a and ID55 showed the highest ones.
The 10 loci allowed the unambiguous discrimination of all accessions.
These markers were also useful in determining the proportion of nucellar and zygotic seedlings for the new CREA rootstocks and helped to identify off-types.
The HRM analysis was simple, fast, and cost-effective and allowed discrimination among rootstocks showing very similar morphological characteristics.
Publication
Authors
M. Cortese, A. Ciacciulli, D. Porrello, D. Pietro Paolo, C. Licciardello, M. Caruso
Keywords
high resolution melting HRM, off-types, polyembryony, INDELs, SNPs
Groups involved
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