Articles
The diversity and transcriptional regulation of limonene biosynthesis in citrus
Article number
1448_43
Pages
335 – 342
Language
English
Abstract
Limonene is a monoterpenoid synthesized by limonene synthase (LS). It is ubiquitous in aromatic plant essential oils but is extremely high in citrus essential oil.
Citrus essential oil is one of most important plant essential oils in the world.
It is composed with a variety of terpene compounds.
The composition of essential oil is varied significantly among the Citrus species and featured with high level of limonene.
Limonene content varies significantly among the different species of citrus, suggesting that the genetic regulation of limonene biosynthesis is highly diverse in citrus.
In this study, we analyzed the sequence structure characteristics and organization of LS orthologous genes in the genome of different species of Citrus subfamily and the correlation with the composition of essential oil.
The relationship between LS orthologous genes and limonene levels in the Citrus subfamily plants at different evolutionary stages showed that limonene levels correspond to the copy number of LS gene, aligning with the evolutionary progression of Citrus subfamily plants.
Transcriptome and metabolome association analysis of CsLS1 overexpression and silence transgenic citrus plants indicated that the contents of limonene and other monoterpenes were significantly altered, indicating that CsLS1 is a key gene in the biosynthesis of monoterpenoids.
Four key transcriptional factors related to CsLS1 were identified.
Our finding provided the preliminary evidence on genetic control of the formation of high level of limonene in citrus and revealed the genetic regulation of the biosynthesis of limonene and other monoterpenoids.
The study laid a foundation for elucidating the genetic mechanism of essential oil biosynthesis and provided genetic resources for directional genetic improvement of essential oil.
Citrus essential oil is one of most important plant essential oils in the world.
It is composed with a variety of terpene compounds.
The composition of essential oil is varied significantly among the Citrus species and featured with high level of limonene.
Limonene content varies significantly among the different species of citrus, suggesting that the genetic regulation of limonene biosynthesis is highly diverse in citrus.
In this study, we analyzed the sequence structure characteristics and organization of LS orthologous genes in the genome of different species of Citrus subfamily and the correlation with the composition of essential oil.
The relationship between LS orthologous genes and limonene levels in the Citrus subfamily plants at different evolutionary stages showed that limonene levels correspond to the copy number of LS gene, aligning with the evolutionary progression of Citrus subfamily plants.
Transcriptome and metabolome association analysis of CsLS1 overexpression and silence transgenic citrus plants indicated that the contents of limonene and other monoterpenes were significantly altered, indicating that CsLS1 is a key gene in the biosynthesis of monoterpenoids.
Four key transcriptional factors related to CsLS1 were identified.
Our finding provided the preliminary evidence on genetic control of the formation of high level of limonene in citrus and revealed the genetic regulation of the biosynthesis of limonene and other monoterpenoids.
The study laid a foundation for elucidating the genetic mechanism of essential oil biosynthesis and provided genetic resources for directional genetic improvement of essential oil.
Publication
Authors
M.Y. Liu, X.F. Liu, Z.M. Ye, W.X. Shen, X.C. Zhao
Keywords
citrus, monoterpene, limonene synthase gene, evolution of species, transcriptional regulation
Groups involved
Online Articles (103)