Articles
Combatting Huanglongbing (HLB) in India: current diagnostics, molecular characterization, and novel management strategies
Article number
1448_63
Pages
497 – 504
Language
English
Abstract
Huanglongbing (HLB), or citrus greening disease, poses a major threat to the global citrus industry.
This disease is caused by a phloem limited bacterium called “Candidatus Liberibacter asiaticus” (CLas) and is spread by psyllids.
Unfortunately, the lack of effective long-term control measures for HLB makes it more challenging to manage.
In recent years, significant progress has been made in detecting HLB using advanced molecular techniques like RPA and LAMP. These methods are praised for their rapid and precise detection capabilities.
We developed a LAMP-based detection assay consisting of specific primer set designed to target the 16S rDNA region of Indian isolates ‘Clas’. Additionally, the HLB-RPA-LFA assay was also developed for on site HLB detection.
To further enhance detection accuracy, an RPA-LFA assay was developed, targeting the CLas five-copy nrdB gene.
Our studies on characterization of CLas revealed greater diversity within ‘Clas’ populations of India compared to Florida and Guangdong.
In India, we found a wider range of patterns in tandem repeats at the genomic locus CLIBASIA_01645, including TRN copy numbers of 9, 10, 11, 12, and 13, with occasional instances of TRN2 and TRN17 patterns.
Notably, northeastern samples exhibited TRN6 and TRN7 patterns, like South China.
Bhutanese CLas isolates were also studied, showing classes II and III at the CLIBASIA_01645 locus, similar to Indian isolates from the northeast, and Term-A, based on the CLIBASIA_05610 locus.
In terms of management practices, several experiments assessed the effectiveness of antimicrobial compounds such as the 2S albumin protein from pumpkin seeds, Nano-ZnO nanoparticle, and inhibitor compounds like pimozide and clidinium bromide, engineered on the cystine binding receptor.
The results demonstrated the efficacy of these compounds in suppressing CLas.
This disease is caused by a phloem limited bacterium called “Candidatus Liberibacter asiaticus” (CLas) and is spread by psyllids.
Unfortunately, the lack of effective long-term control measures for HLB makes it more challenging to manage.
In recent years, significant progress has been made in detecting HLB using advanced molecular techniques like RPA and LAMP. These methods are praised for their rapid and precise detection capabilities.
We developed a LAMP-based detection assay consisting of specific primer set designed to target the 16S rDNA region of Indian isolates ‘Clas’. Additionally, the HLB-RPA-LFA assay was also developed for on site HLB detection.
To further enhance detection accuracy, an RPA-LFA assay was developed, targeting the CLas five-copy nrdB gene.
Our studies on characterization of CLas revealed greater diversity within ‘Clas’ populations of India compared to Florida and Guangdong.
In India, we found a wider range of patterns in tandem repeats at the genomic locus CLIBASIA_01645, including TRN copy numbers of 9, 10, 11, 12, and 13, with occasional instances of TRN2 and TRN17 patterns.
Notably, northeastern samples exhibited TRN6 and TRN7 patterns, like South China.
Bhutanese CLas isolates were also studied, showing classes II and III at the CLIBASIA_01645 locus, similar to Indian isolates from the northeast, and Term-A, based on the CLIBASIA_05610 locus.
In terms of management practices, several experiments assessed the effectiveness of antimicrobial compounds such as the 2S albumin protein from pumpkin seeds, Nano-ZnO nanoparticle, and inhibitor compounds like pimozide and clidinium bromide, engineered on the cystine binding receptor.
The results demonstrated the efficacy of these compounds in suppressing CLas.
Publication
Authors
D.K. Ghosh, A.K. Sharma, S. Santra
Keywords
HLB pandemic, CLIBASIA_01645, molecular detection, RPA, LAMP and 2S albumin and Nano-ZnO formulation
Groups involved
Online Articles (103)