Articles
COMPARISON OF HIGH AND LOW QUALITY PEACH FRUIT CULTIVARS BY DIFFERENTIAL HYBRIDIZATION OF A CDNA LIBRARY
Article number
254_7
Pages
48 – 48
Language
Abstract
We are studying genes that are expressed during peach fruit development.
The object of this work was to isolate genes whose levels of expression differ during fruit development between the high fruit quality cultivar ‘Suncrest’ and the low fruit quality cultivar ‘Bailey’. RNA populations from the two cultivars during fruit development were compared by in vitro translation.
The most differences in RNA populations between the two cultivars were apparent 30 days after bloom.
A cDNA library (a collection of cDNA clones from a particular mRNA population) was constructed in a lambdoid vector from ‘Suncrest’ fruit RNA collected thirty days after bloom.
This cDNA library was screened in duplicate with single stranded radioactive cDNAs derived from ‘Suncrest’ and ‘Bailey’ fruit tissue.
Five clones that preferentially hybridized to the RNAs from ‘Suncrest’ were further purified and isolated.
Dot blot analyses indicate that the expression of the most highly expressed cloned sequence is up to five times greater in ‘Suncrest’ than in ‘Bailey’. We are currently determining the amount and time of expression of these five cloned sequences in developing fruit from six different cultivars.
The object of this work was to isolate genes whose levels of expression differ during fruit development between the high fruit quality cultivar ‘Suncrest’ and the low fruit quality cultivar ‘Bailey’. RNA populations from the two cultivars during fruit development were compared by in vitro translation.
The most differences in RNA populations between the two cultivars were apparent 30 days after bloom.
A cDNA library (a collection of cDNA clones from a particular mRNA population) was constructed in a lambdoid vector from ‘Suncrest’ fruit RNA collected thirty days after bloom.
This cDNA library was screened in duplicate with single stranded radioactive cDNAs derived from ‘Suncrest’ and ‘Bailey’ fruit tissue.
Five clones that preferentially hybridized to the RNAs from ‘Suncrest’ were further purified and isolated.
Dot blot analyses indicate that the expression of the most highly expressed cloned sequence is up to five times greater in ‘Suncrest’ than in ‘Bailey’. We are currently determining the amount and time of expression of these five cloned sequences in developing fruit from six different cultivars.
Publication
Authors
A. Callahan, P. Morgens, R. Scorza, S. Mante, J. Cordts, R. Cohen
Keywords
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