Articles
PROTOPLAST TECHNOLOGY AND REGENERATION STUDIES FOR RUBUS BREEDING
Article number
505_27
Pages
215 – 222
Language
Abstract
Efficient protocols for protoplast isolation and culture from leaf tissue and cell suspensions of cv. ‘Autumn Bliss’, a primocane fruiting (PF) raspberry (2n=2x=14), and of cv.’Hull Thornless’, a thornfree blackberry (2n=4x=28) were developed following studies on the effect of different pre-treatments and digestion solutions.
Protoplast suspensions of these two species (Autumn Bliss and Hull Thornless) were fused following different durations of polyethylene glycol (PEG) treatments.
Duration of PEG treatment and subsequent culture media influenced the rate of fusion cell division.
Colony formation was initiated on solid media and hexaploid calli were selected after 3 to 4 months.
Heterokarion origin of hexaploid callus lines were identified among fusion products and were studied using RAPD molecular marker techniques in conjunction with genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). Plant regeneration from the fusion calli under different combinations of Plant Growth Regulator (PGR) and stress treatments, were initiated.
Root formation and pre-embryonic growth were the only structural differentiation attained from these somatic hybrid callus lines.
Studies of the mechanisms regulating cell differentiation were carried out with the aim to improve the regeneration response.
The changes in cell content of specific compounds, such as endogenous nucleotides, are suspected to play a possible role in cell differentiation.
Preliminary results indicate the importance of these compounds in the regulation of Rubus cell division and differentiation.
Protoplast suspensions of these two species (Autumn Bliss and Hull Thornless) were fused following different durations of polyethylene glycol (PEG) treatments.
Duration of PEG treatment and subsequent culture media influenced the rate of fusion cell division.
Colony formation was initiated on solid media and hexaploid calli were selected after 3 to 4 months.
Heterokarion origin of hexaploid callus lines were identified among fusion products and were studied using RAPD molecular marker techniques in conjunction with genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). Plant regeneration from the fusion calli under different combinations of Plant Growth Regulator (PGR) and stress treatments, were initiated.
Root formation and pre-embryonic growth were the only structural differentiation attained from these somatic hybrid callus lines.
Studies of the mechanisms regulating cell differentiation were carried out with the aim to improve the regeneration response.
The changes in cell content of specific compounds, such as endogenous nucleotides, are suspected to play a possible role in cell differentiation.
Preliminary results indicate the importance of these compounds in the regulation of Rubus cell division and differentiation.
Publication
Authors
B. Mezzetti, L. Landi, H.B. Phan, I. Mantovani, S. Ruggieri, P. Rosati, K.Y. Lim
Keywords
Online Articles (61)