NITRATE AND CHLORIDE TRANSPORT INTERACTION IN GRAPEVINE

The effect of nitrate and chloride on the ion transport of rootstock varieties (Fercal, Rupestris du Lot, Georgikon 28) and a scion Kahet was examined with 25 mM Ca(NO₃)₂ or Ca(Cl)₂ treatment of rooted one node cuttings grown in 1/10 Hoagland solution.
Roots and leaves were analysed for water extractable fraction of anions (NO₃^–, Cl^–, HPO₄^2-, SO₄^2-; HPLC method) and cations (K⁺, Ca²⁺, Mg²⁺, Na⁺, AAS method). Varietal differences in anion content of the roots were significant: 5–10 times higher anion levels were measured in Georgikon 28 and Rupestris du Lot than in Kahet and Fercal and this difference was smaller in the leaf blades.
Cl^– was the most mobile anion in the plants reflected by the high Cl^– level of the roots and leaves in Cl^– treatments.
Two different defending mechanisms can be distinguished on the basis of Cl- content of the roots: Cl^– accumulation (Georgikon 28 and Rupestris du Lot, 2331 and 1845 μmol/gdw.
Cl^– respectively) and Cl^– exclusion (Kahet and Fercal, 230 and 518 μmol/gdw.
Cl^–).. A mutual interference of Cl^– and NO₃^– ions could be observed.
Elevated level (50 mM) of Cl^– or NO₃^– in the nutrient solution inhibited the transport of the other anion to a large extent.
In Cl^– treatments the NO₃^– concentration of the roots was decreased to 30–60% of the NO₃^– content of the control plants.
In the presence of 50 mM NO₃^–, Cl^– was not found in the roots of Rupestris du Lot and Georgikon 28 and in Kahet and Fercal the low control Cl^– level was slightly affected.
In 25 mM Ca(Cl)₂ treatments the intensive Cl^– transport was accompanied by the increase of the water soluble K⁺, Ca²⁺ and Mg²⁺ concentration in the leaves.

V International Symposium on Grapevine Physiology

E. Miklós, Zs. Szegletes, L. Erdei

nitrate, chloride, grapevine variety, ion transport

https://doi.org/10.17660/ActaHortic.2000.526.25