Articles
BETA-1,3-GLUCANASE MRNA SYNTHESIS IN GRAPEVINE LEAVES AFTER BOTRYTIS INOCULATION, AND IN BERRIES AFTER WOUNDING OR DURING RIPENING
Article number
526_47
Pages
429 – 436
Language
English
Abstract
The hydrolytic enzymes beta-1,3-glucanases (EC 3.2.1.39) are known to be involved in plant defense reactions against pathogens or in developmental events.
Transcription of beta-1,3-glucanase gene was studied in grapevine (Vitis vinifera L.): in leaves after induction with Botrytis cinerea, or in berries after mechanical wounding; and during berry development.
Northern blot hybridization showed that beta-1,3-glucanase mRNA strongly accumulated in grapevine leaves following infection with Botrytis. The transcript was detectable at 2 days after infection, and reached a maximum level at 4 days post-infection.
During berries development, no constitutive expression was observed during their green growth period (before ripening), whereas the synthesis of
-1,3-glucanase was detected during fruit ripening.
On the contrary, mRNA of this isoform was induced over fruit green growth period in response to wounding treatments.
Transcription of beta-1,3-glucanase gene was studied in grapevine (Vitis vinifera L.): in leaves after induction with Botrytis cinerea, or in berries after mechanical wounding; and during berry development.
Northern blot hybridization showed that beta-1,3-glucanase mRNA strongly accumulated in grapevine leaves following infection with Botrytis. The transcript was detectable at 2 days after infection, and reached a maximum level at 4 days post-infection.
During berries development, no constitutive expression was observed during their green growth period (before ripening), whereas the synthesis of
-1,3-glucanase was detected during fruit ripening.On the contrary, mRNA of this isoform was induced over fruit green growth period in response to wounding treatments.
Authors
E. Kraeva, A. Renault, N. Terrier, C. Tesnière, C. Romieu, F. Sauvage, J. Bierne, A. Deloire
Keywords
Beta-1,3-glucanase, pathogenesis-related proteins, development, leaves, berries, grapevine
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