CLONAL MICROPROPAGATION OF CULTIVAR BOLGAR (VITIS VINIFERA L. SSP. SATIVA) AND NUTRIENT MEDIUM COMPOSITION OPTIMIZATION: A MATHEMATICAL APPROACH.

The problem of the clonal micropropagation possibilities as an intensive method for vine propagation is still a live question yet unsolved and it is the subject of study by a number of researchers: Brendel (1986); Reisch (1986); Barlass et al. (1978); Silvestroni (1981); Galzy (1990); Fallot et al. (1992); Marten et al. (1992). Most of them have improved the morphogenesis medium for in vitro vine propagation in an experimental way: Harris et al. (1982); Reuther (1983); Chee et al. (1985), (1988); Hassani (1990); Lee et al. (1990). According to Shamina et al. (1976) the methods of the mathematical planning were one of the most reliable for defining the cultivation conditions as they not only showed which medium components influenced positively the morphogenesis but showed the optimum relation of these factors.
We were not aware of studies concerning the improvement of the cultivation conditions applying the mathematical planning methods for vine propagation in vitro.
Thus our aim was to study the possibility for obtaining of maximum number of microcuttings at clonal micropropagation of cv.
Bolgar by means of optimizing the nutrient medium composition depending on the biological peculiarities of the cultivar with a mathematical approach.
The idea for this study were the results obtained by us, Dimitrova et al., (1992), proving the varietal specificity for vine cultivation in vitro.

V International Symposium on Grapevine Physiology

V. Dimitrova

https://doi.org/10.17660/ActaHortic.2000.526.30